Journal article
A multiplex PCR test for determination of mating type applied to the plant pathogens Tapesia yallundae and Tapesia acuformis
Fungal genetics and biology, Vol.33(3), pp.173-180
08/2001
Handle:
https://hdl.handle.net/2376/107959
PMID: 11495574
Abstract
A multiplex PCR test for determining mating type of the pathogens Tapesia yallundae and Tapesia acuformis is described. The test involves three primers: a "common" primer annealing to DNA sequence conserved in the flanking region of both mating-type idiomorphs and two specific primers annealing to sequence in either the MAT-1 or the MAT-2 idiomorphs. Locating the specific primers in different positions relative to the common primer yielded PCR products of 812 or 418 bp from MAT-1 and MAT-2 isolates, respectively. The test was used successfully to determine the mating type of 118 isolates of T. yallundae and T. acuformis from Europe, North America, and New Zealand. Isolates of both mating types were found on all continents for both species despite the rarely observed occurrence of sexual reproduction of T. acuformis. The multiplex test design should be applicable to other ascomycete species, of use in studies of MAT distribution and facilitating sexual crossing by identifying compatible isolates.
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Details
- Title
- A multiplex PCR test for determination of mating type applied to the plant pathogens Tapesia yallundae and Tapesia acuformis
- Creators
- P S Dyer - School of Life and Environmental Sciences, The University of Nottingham, Nottingham, NG7 2RD, United Kingdom. Paul.Dyer@Nottingham.ac.ukP A FurneauxG DouhanT D Murray
- Publication Details
- Fungal genetics and biology, Vol.33(3), pp.173-180
- Academic Unit
- Plant Pathology, Department of
- Publisher
- United States
- Identifiers
- 99900546739601842
- Language
- English
- Resource Type
- Journal article