Journal article
A new fabrication technique to form complex polymethylmethacrylate microchannel for bioseparation
Biomicrofluidics, Vol.6(1), pp.016503-016503-13
03/2012
Handle:
https://hdl.handle.net/2376/108920
PMCID: PMC3293388
PMID: 22396722
Abstract
Recent studies show that reduction in cross-sectional area can be used to improve the concentration factor in microscale bioseparations. Due to simplicity in fabrication process, a step reduction in cross-sectional area is generally implemented in microchip to increase the concentration factor. But the sudden change in cross-sectional area can introduce significant band dispersion and distortion. This paper reports a new fabrication technique to form a gradual reduction in cross-sectional area in polymethylmethacrylate (PMMA) microchannel for both anionic and cationic isotachophoresis (ITP). The fabrication technique is based on hot embossing and surface modification assisted bonding method. Both one-dimensional and two-dimensional gradual reduction in cross-sectional area microchannels were formed on PMMA with high fidelity using proposed techniques. ITP experiments were conducted to separate and preconcentrate fluorescent proteins in these microchips. Thousand fold and ten thousand fold increase in concentrations were obtained when 10 × and 100 × gradual reduction in cross-sectional area microchannels were used for ITP.
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Details
- Title
- A new fabrication technique to form complex polymethylmethacrylate microchannel for bioseparation
- Creators
- Talukder Z Jubery - Mechanical and Materials Engineering, Washington State University, Pullman, Washington 99164, USAMohammad R Hossan - Mechanical and Materials Engineering, Washington State University, Pullman, Washington 99164, USADanny R Bottenus - Chemical Engineering and Bioengineering, Washington State University, Pullman, Washington 99164, USACornelius F Ivory - Chemical Engineering and Bioengineering, Washington State University, Pullman, Washington 99164, USAWenji Dong - Chemical Engineering and Bioengineering, Washington State University, Pullman, Washington 99164, USAPrashanta Dutta - Mechanical and Materials Engineering, Washington State University, Pullman, Washington 99164, USA
- Publication Details
- Biomicrofluidics, Vol.6(1), pp.016503-016503-13
- Academic Unit
- School of Chemical Engineering and Bioengineering; School of Mechanical and Materials Engineering
- Publisher
- American Institute of Physics
- Identifiers
- 99900547371501842
- Language
- English
- Resource Type
- Journal article