A novel ARID DNA-binding protein interacts with SymRK and is expressed during early nodule development in Lotus japonicus

Hui Zhu; Tao Chen; Maosheng Zhu; Qing Fang; Heng Kang; Zonglie Hong; Zhongming Zhang

doi:10.1104/pp.108.119164

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A novel ARID DNA-binding protein interacts with SymRK and is expressed during early nodule development in Lotus japonicus

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A novel ARID DNA-binding protein interacts with SymRK and is expressed during early nodule development in Lotus japonicus

Hui Zhu, Tao Chen, Maosheng Zhu, Qing Fang, Heng Kang, Zonglie Hong and Zhongming Zhang

Plant physiology (Bethesda), Vol.148(1), pp.337-347

09/2008

DOI: https://doi.org/10.1104/pp.108.119164

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https://hdl.handle.net/2376/102981

PMID: 18633121

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Abstract

Amino Acid Sequence

Promoter Regions, Genetic

Gene Expression

Transcriptional Activation

Loteae - metabolism

Root Nodules, Plant - microbiology

Molecular Sequence Data

Loteae - microbiology

DNA - metabolism

DNA-Binding Proteins - metabolism

Transcription Factors - metabolism

Loteae - growth & development

Plant Proteins - metabolism

Protein Interaction Domains and Motifs

Root Nodules, Plant - growth & development

Dimerization

During the establishment of symbiosis in legume roots, the rhizobial Nod factor signal is perceived by the host cells via receptor-like kinases, including SymRK. The NODULE INCEPTION (NIN) gene in Lotus japonicus is required for rhizobial entry into root cells and for nodule organogenesis. We describe here a novel DNA-binding protein from L. japonicus, referred to as SIP1, because it was identified as a SymRK-interacting protein. SIP1 contains a conserved AT-rich interaction domain (ARID) and represents a unique member of the ARID-containing proteins in plants. The C terminus of SIP1 was found to be responsible for its interaction with the kinase domain of SymRK and for homodimerization in the absence of DNA. SIP1 specifically binds to the promoter of LjNIN but not to that of LjCBP1 (a calcium-binding protein gene), both of which are known to be inducible by Nod factors. SIP1 recognizes two of the three AT-rich domains present in the NIN gene promoter. Deletion of one of the AT-rich domains at the NIN promoter diminishes the binding of SIP1 to the NIN promoter. The protein is localized to the nuclei when expressed as a red fluorescence fusion protein in the onion (Allium cepa) epidermal cells. The SIP1 gene is expressed constitutively in the uninfected roots, and its expression levels are elevated after infection by Mesorhizobium loti. It is proposed that SIP1 may be required for the expression of NIN and involved in the initial communications between the rhizobia and the host root cells.

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Title: A novel ARID DNA-binding protein interacts with SymRK and is expressed during early nodule development in Lotus japonicus
Creators: Hui Zhu - State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China
Tao Chen
Maosheng Zhu
Qing Fang
Heng Kang
Zonglie Hong
Zhongming Zhang
Publication Details: Plant physiology (Bethesda), Vol.148(1), pp.337-347
Academic Unit: Center for Reproductive Biology; Crop and Soil Sciences, Department of
Publisher: United States
Identifiers: 99900546509101842
Language: English
Resource Type: Journal article