Journal article
A novel continuous centrifugal bioreactor for high‐density cultivation of mammalian and microbial cells
Biotechnology and bioengineering, Vol.38(10), pp.1190-1202
12/05/1991
Handle:
https://hdl.handle.net/2376/105229
PMID: 18600715
Abstract
A continuous centrifugal bioreactor (CCBR), developed to study the growth and productivity of dense suspensions cultures, has been applied to both fermentation and mammalian cell cultivation processes. With this approach, high‐density nonflocculent cultures are maintained in a tapered fluidized bed by balancing the drag forces on the cells due to following substrate with the centrifugal forces. The Sysyem was first used to produce ethanol by fermentation with Saccharomyces cerevisiae; then with H21A1 mouse hybridoma cells secreting monoclonal antibody (MoAb), lgM. Results of this research show the feasibility of using the CCBR for both production of secreted products and as a research tool for studying cell metabolism and production kinetics. Media recycle may be used to modify the behavior of the system form a plug flow apparatus to a continuous stirred reactor (CSTR).
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Details
- Title
- A novel continuous centrifugal bioreactor for high‐density cultivation of mammalian and microbial cells
- Creators
- Bernard J Van WieThomas M BrounsMichael L ElliotWilliam C Davis
- Publication Details
- Biotechnology and bioengineering, Vol.38(10), pp.1190-1202
- Academic Unit
- Veterinary Microbiology and Pathology, Department of; Chemical Engineering and Bioengineering, School of
- Publisher
- Wiley Subscription Services, Inc., A Wiley Company; Hoboken
- Number of pages
- 13
- Identifiers
- 99900546945001842
- Language
- English
- Resource Type
- Journal article