Journal article
A novel immunochromatographic electrochemical biosensor for highly sensitive and selective detection of trichloropyridinol, a biomarker of exposure to chlorpyrifos
Biosensors & bioelectronics, Vol.26(6), pp.2835-2840
02/15/2011
Handle:
https://hdl.handle.net/2376/110564
PMID: 21195597
Abstract
We present a novel portable immunochromatographic electrochemical biosensor (IEB) for simple, rapid, and sensitive biomonitoring of trichloropyridinol (TCP), a metabolite biomarker of exposure to organophosphorus insecticides. Our new approach takes the advantage of immunochromatographic test strip for a rapid competitive immunoreaction and a disposable screen-printed carbon electrode for a rapid and sensitive electrochemical analysis of captured HRP labeling. Several key experimental parameters (e.g. immunoreaction time, the amount of HRP labeled TCP, concentration of the substrate for electrochemical measurements, and the blocking agents for the nitrocellulose membrane) were optimized to achieve a high sensitivity, selectivity and stability. Under optimal conditions, the IEB has demonstrated a wide linear range (0.1–100ng/ml) with a detection limit as low as 0.1ng/ml TCP. Furthermore, the IEB has been successfully applied for biomonitoring of TCP in the rat plasma samples with in vivo exposure to organophosphorus insecticides like Chlorpyrifos-oxon (CPF-oxon). The IEB thus opens up new pathways for designing a simple, rapid, clinically accurate, and quantitative tool for TCP detection, as well as holds a great promise for in-field screening of metabolite biomarkers, e.g., TCP, for humans exposed to organophosphorus insecticides.
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Details
- Title
- A novel immunochromatographic electrochemical biosensor for highly sensitive and selective detection of trichloropyridinol, a biomarker of exposure to chlorpyrifos
- Creators
- Limin Wang - Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects, Ministry of Agriculture, Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, ChinaDonglai Lu - Pacific Northwest National Laboratory, Richland, WA 99352, United StatesJun Wang - Pacific Northwest National Laboratory, Richland, WA 99352, United StatesDan Du - Pacific Northwest National Laboratory, Richland, WA 99352, United StatesZhexiang Zou - Pacific Northwest National Laboratory, Richland, WA 99352, United StatesHua Wang - Pacific Northwest National Laboratory, Richland, WA 99352, United StatesJordan N Smith - Pacific Northwest National Laboratory, Richland, WA 99352, United StatesCharles Timchalk - Pacific Northwest National Laboratory, Richland, WA 99352, United StatesFengquan Liu - Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects, Ministry of Agriculture, Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, ChinaYuehe Lin - Pacific Northwest National Laboratory, Richland, WA 99352, United States
- Publication Details
- Biosensors & bioelectronics, Vol.26(6), pp.2835-2840
- Academic Unit
- School of Mechanical and Materials Engineering
- Publisher
- Elsevier B.V
- Identifiers
- 99900547038701842
- Language
- English
- Resource Type
- Journal article