A pre-immediate-early role for tegument ICP0 in the proteasome-dependent entry of herpes simplex virus

Mark G Delboy; Anthony V Nicola

doi:10.1128/JVI.00267-11

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A pre-immediate-early role for tegument ICP0 in the proteasome-dependent entry of herpes simplex virus

Journal article

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Peer reviewed

A pre-immediate-early role for tegument ICP0 in the proteasome-dependent entry of herpes simplex virus

Mark G Delboy and Anthony V Nicola

Journal of virology, Vol.85(12), pp.5910-5918

06/2011

DOI: https://doi.org/10.1128/JVI.00267-11

Handle:

https://hdl.handle.net/2376/109114

PMCID: PMC3126318

PMID: 21471243

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Abstract

Cell Line

Cricetinae

Cricetulus

Humans

Ubiquitin-Protein Ligases - metabolism

Cercopithecus aethiops

Virion - metabolism

Virus Internalization

Immediate-Early Proteins - metabolism

Herpesvirus 1, Human - physiology

Animals

Immediate-Early Proteins - genetics

Herpesvirus 1, Human - genetics

Proteasome Endopeptidase Complex - metabolism

Capsid - metabolism

Ubiquitin-Protein Ligases - genetics

Vero Cells

Herpesvirus 1, Human - pathogenicity

CHO Cells

Herpes simplex virus (HSV) entry requires host cell 26S proteasomal degradation activity at a postpenetration step. When expressed in the infected cell, the HSV immediate-early protein ICP0 has E3 ubiquitin ligase activity and interacts with the proteasome. The cell is first exposed to ICP0 during viral entry, since ICP0 is a component of the inner tegument layer of the virion. The function of tegument ICP0 is unknown. Deletion of ICP0 or mutations in the N-terminal RING finger domain of ICP0 results in the absence of ICP0 from the tegument. We show here that these mutations negatively influenced the targeting of incoming capsids to the nucleus. Inhibitors of the chymotrypsin-like activity of the proteasome the blocked entry of virions containing tegument ICP0, including ICP0 mutants that are defective in USP7 binding. However, ICP0-deficient virions were not blocked by proteasomal inhibitors and entered cells via a proteasome-independent mechanism. ICP0 appeared to play a postpenetration role in cells that supported either endocytosis or nonendosomal entry pathways for HSV. The results suggest that ICP0 mutant virions are defective upstream of viral gene expression at a pre-immediate-early step in infection. We propose that proteasome-mediated degradation of a virion or host protein is regulated by ICP0 to allow efficient delivery of entering HSV capsids to the nuclear periphery.

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Title: A pre-immediate-early role for tegument ICP0 in the proteasome-dependent entry of herpes simplex virus
Creators: Mark G Delboy - Department of Microbiology and Immunology, Virginia Commonwealth University, School of Medicine, Richmond, Virginia 23298-0678, USA
Anthony V Nicola
Publication Details: Journal of virology, Vol.85(12), pp.5910-5918
Academic Unit: Veterinary Microbiology and Pathology, Department of
Publisher: United States
Grant note: AI-083850 / NIAID NIH HHS R21 AI083850 / NIAID NIH HHS
Identifiers: 99900547299101842
Language: English
Resource Type: Journal article