Journal article
A rapid, reliable method of evaluating growth and viability of intraerythrocytic protozoan hemoparasites using fluorescence flow cytometry
Memórias do Instituto Oswaldo Cruz, Vol.87(suppl 3), pp.235-239
01/1992
Handle:
https://hdl.handle.net/2376/117736
PMID: 1343696
Abstract
Fluorescence flow cytometry was employed to assess the potential of a vital dye, hydroethiedine, for use in the detection and monitoring of the viability of hemoparasites in infected erythrocytes, using Babesia bovis as a model parasite. The studies demonstrated that hydroethidine is taken up by B. bovis and metabolically converted to the DNA binding fluorochrone, ethidium. Following uptake of the dye, erythrocytes contamine viable parasites were readily distinguished and quantitated. Timed studies with the parasiticidal drug, Ganaseg, showed that it is possible to use the fluorochrome assay to monitor the effects of the drug on the rate of replication and viability of B. bovis in culture. The assay provides a rapid method for evaluation of the in vitro effect of drugs on hemoparasites and for analysis of the effect of various components of the immune response, such as lymphokines, monocyte products, antibodies, and effector cells (T, NK, LAK, ADCC) on the growth and viability of intraerythrocytic parasites.
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Details
- Title
- A rapid, reliable method of evaluating growth and viability of intraerythrocytic protozoan hemoparasites using fluorescence flow cytometry
- Creators
- W. C DavisC. R WyattM. J HamiltonW. L Goff - Washington State University
- Publication Details
- Memórias do Instituto Oswaldo Cruz, Vol.87(suppl 3), pp.235-239
- Academic Unit
- Veterinary Microbiology and Pathology, Department of
- Publisher
- Instituto Oswaldo Cruz, Ministério da Saúde
- Identifiers
- 99900548145301842
- Language
- Portuguese
- Resource Type
- Journal article