Journal article
A selective phosphine-based fluorescent probe for nitroxyl in living cells
Bioorganic & medicinal chemistry letters, Vol.25(1), pp.16-19
01/01/2015
Handle:
https://hdl.handle.net/2376/113888
PMCID: PMC4355083
PMID: 25465170
Abstract
[Display omitted]
A novel fluorescein-based fluorescent probe for nitroxyl (HNO) based on the reductive Staudinger ligation of HNO with an aromatic phosphine was prepared. This probe reacts with HNO derived from Angeli’s salt and 4-bromo Piloty’s acid under physiological conditions without interference by other biological redox species. Confocal microscopy demonstrates this probe detects HNO by fluorescence in HeLa cells and mass spectrometric analysis of cell lysates confirms this probe detects HNO following the proposed mechanism.
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Details
- Title
- A selective phosphine-based fluorescent probe for nitroxyl in living cells
- Creators
- Zhengrui Miao - Department of Chemistry, Wake Forest University, Winston-Salem, NC 27109, United StatesJulie A Reisz - Department of Chemistry, Wake Forest University, Winston-Salem, NC 27109, United StatesSusan M Mitroka - Department of Chemistry, Wake Forest University, Winston-Salem, NC 27109, United StatesJia Pan - Department of Chemistry, Washington State University, Pullman, WA 99164, United StatesMing Xian - Department of Chemistry, Washington State University, Pullman, WA 99164, United StatesS. Bruce King - Department of Chemistry, Wake Forest University, Winston-Salem, NC 27109, United States
- Publication Details
- Bioorganic & medicinal chemistry letters, Vol.25(1), pp.16-19
- Academic Unit
- Chemistry, Department of
- Publisher
- Elsevier Ltd
- Identifiers
- 99900548253801842
- Language
- English
- Resource Type
- Journal article