Journal article
A "sense-and-treat" ELISA using zeolitic imidazolate framework-8 as carriers for dual-modal detection of carcinoembryonic antigen
Sensors and actuators. B, Chemical, Vol.297, p.126760
10/15/2019
Abstract
Enzyme-linked immunosorbent assay (ELISA) has been widely used in the area of biological analysis, while there are still some challenges getting in the way to ensure both excellent sensitivity and reliable accuracy. To overcome these obstacles, we simulated the "smart" sense-and-treat carriers in a drug delivery system to fabricate an improved ELISA, employing zeolitic imidazolate framework-8 (ZIF-8) as the carrier to deliver the tracer agent carbon dots (CDs) and the "drug" thymolphthalein (TP), which was referred to as a "sense-and-treat" ELISA. In the "sense" part, the strong fluorescence intensity of CDs could be observed directly to achieve the sensitive detection of the target. In the "treat" part, after stimulation of alkaline solution, TP was released from ZIF-8 carriers and generated a color change with an obvious absorption, which was beneficial for increasing the sensitivity of this ELISA due to the high loading of the TP. Finally, we took carcinoembryonic antigen (CEA) as a model and performed dual-modal detection using this improved "sense-and-treat" ELISA to accomplish quantitative determinations sensitively and accurately. In addition, the results indicated by the two parts kept good concordance, which could demonstrate the reliable accuracy of this ELISA.
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Details
- Title
- A "sense-and-treat" ELISA using zeolitic imidazolate framework-8 as carriers for dual-modal detection of carcinoembryonic antigen
- Creators
- Hongye Yan - Central China Normal UniversityLei Jiao - Central China Normal UniversityHengjia Wang - Central China Normal UniversityWeiqing Xu - Central China Normal UniversityYu Wu - Central China Normal UniversityWenling Gu - Central China Normal UniversityDan Du - Washington State UniversityYuehe Lin - Washington State UniversityChengzhou Zhu - Central China Normal University
- Publication Details
- Sensors and actuators. B, Chemical, Vol.297, p.126760
- Academic Unit
- School of Mechanical and Materials Engineering
- Publisher
- Elsevier
- Number of pages
- 7
- Grant note
- Recruitment Program of Global Youth Experts of China CCNU18JCXK07 / self-determined research funds of CCNU from the college's basic research and operation of MOE
- Identifiers
- 99901227840401842
- Language
- English
- Resource Type
- Journal article