Journal article
A simple methodology for rate-limiting step determination for anaerobic digestion of complex substrates and effect of microbial community ratio
Bioresource technology, Vol.134, pp.391-395
04/2013
Handle:
https://hdl.handle.net/2376/106555
PMID: 23489573
Abstract
► A method was developed to evaluate limiting steps for complex substrate digestion. ► The microbial community ratio concept was proposed and determined by ATP analysis. ► The effect of microbial community ratio on kinetics and limiting step was studied.
Anaerobic digestion (AD) of complex substrates is a multi-step process, which is kinetically controlled by an individual rate-limiting step. A methodology for determining the rate-limiting step during AD of complex substrates was developed by supplementation of metabolic intermediates from each digestion step with dairy manure as an emblematic complex substrate. This method elucidated that hydrolysis of dairy manure was the rate-limiting step when normal anaerobic sludge was used as inoculum. Furthermore, the concept and effect of microbial community ratio was introduced by manipulating two different inocula, i.e. normal anaerobic sludge and heated anaerobic sludge, so that varying ratios (r) of hydrolytic and methanogenic bacteria could be studied. Results revealed that the rate-limiting step changed with the variation of r. For dairy manure, results indicated a critical ratio r∗=24 between hydrolytic bacteria and methanogens, whereby as r decreased or exceeded from this value, hydrolysis or methanogenesis limited the AD process, respectively.
Metrics
11 Record Views
Details
- Title
- A simple methodology for rate-limiting step determination for anaerobic digestion of complex substrates and effect of microbial community ratio
- Creators
- Jingwei Ma - Department of Biological Systems Engineering, Washington State University, Pullman, WA 99164, USACraig Frear - Department of Biological Systems Engineering, Washington State University, Pullman, WA 99164, USAZhi-wu Wang - Department of Biological Systems Engineering, Washington State University, Pullman, WA 99164, USALiang Yu - Department of Biological Systems Engineering, Washington State University, Pullman, WA 99164, USAQuanbao Zhao - Center for Resources and Environmental Research, Beijing University of Chemical Technology, Beijing 100029, ChinaXiujin Li - Center for Resources and Environmental Research, Beijing University of Chemical Technology, Beijing 100029, ChinaShulin Chen - Department of Biological Systems Engineering, Washington State University, Pullman, WA 99164, USA
- Publication Details
- Bioresource technology, Vol.134, pp.391-395
- Academic Unit
- Biological Systems Engineering, Department of; Chemistry, Department of
- Publisher
- Elsevier Ltd
- Identifiers
- 99900547074701842
- Language
- English
- Resource Type
- Journal article