Journal article
A simple two-step method for the conversion of [ 3H]cortisol to [ 3H]-11-ketotestosterone
Steroids, Vol.62(10), pp.655-658
1997
Handle:
https://hdl.handle.net/2376/112953
PMID: 9381512
Abstract
Despite the existence of several protocols, problems appear to persist in the small scale chemical synthesis of radiolabeled 11-ketotestosterone from cortisol. We investigated the possibilities of using the mild oxidant pyridinium dichromate for the oxidative cleavage of the dihydroxyacetone side chain of cortisol and 17β-hydroxysteroid dehydrogenase for the subsequent reduction of the resulting 17-keto group. Our protocol has resulted in consistently high yields of both the intermediate, adrenosterone (70–80%), and the product, 11-ketotestosterone (up to 60%). This, taken together with the convenience and relatively low cost of our method, recommends the protocol for its use for the synthesis of [
3H]-11-ketotestosterone for endocrine studies.
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Details
- Title
- A simple two-step method for the conversion of [ 3H]cortisol to [ 3H]-11-ketotestosterone
- Creators
- P.Mark Lokman - Department of Zoology, University of Otago, Dunedin, New ZealandL Irwin - Department of Chemistry, Massey University, Palmerston-North, New ZealandLeonard F Blackwell - Department of Chemistry, Massey University, Palmerston-North, New ZealandPeter S Davie - Department of Physiology and Anatomy, Massey University, Palmerston-North, New ZealandMervyn Thomas - Department of Chemistry, University of Otago, Dunedin, New ZealandGraham Young - Department of Zoology, University of Otago, Dunedin, New Zealand
- Publication Details
- Steroids, Vol.62(10), pp.655-658
- Academic Unit
- UNKNOWN
- Publisher
- Elsevier Inc
- Identifiers
- 99900547997501842
- Language
- English
- Resource Type
- Journal article