Journal article
A simplified QTL mapping approach for screening and mapping of novel AFLP markers associated with beef marbling
Journal of biotechnology, Vol.127(2), pp.177-187
2007
Handle:
https://hdl.handle.net/2376/101088
PMID: 16901568
Abstract
Genome screening of quantitative trait loci (QTL) for a complex trait is usually costly and highly laborious, as it requires a large number of markers spanning the whole genome. Here we present a simplified approach for screening and mapping of QTL-linked markers for beef marbling using a Wagyu
×
Limousin F
2 reference population. This simplified approach involves integration of the amplified fragment length polymorphism (AFLP) with DNA pooling and selective genotyping and comparative bioinformatics tools. AFLP analysis on two high and two low marbling DNA pools yielded ten visually different markers. Among them, four were confirmed based on individual AFLP validation. Sequencing and
in silico characterization assigned two of these AFLP markers to bovine chromosomes 1 (BTA1) and 13 (BTA13), which are orthologous to human chromosomes HSA21q22.2 and HSA10p11.23 with both regions harboring QTL for obesity-related phenotypes. Both AFLP markers showed significantly large additive genetic effects (0.28
±
0.11 on BTA1 and 0.54
±
0.21 on BTA13) on beef-marbling score (BMS) (
P
<
0.05). Overall, this approach is less time consuming, inexpensive and in particular, suitable for screening and mapping QTL-linked markers when targeting one or a few complex traits.
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Details
- Title
- A simplified QTL mapping approach for screening and mapping of novel AFLP markers associated with beef marbling
- Creators
- Qianjun Xiao - Department of Animal Sciences, Washington State University, Pullman, WA 99164-6351, USATito A Wibowo - Department of Animal Sciences, Washington State University, Pullman, WA 99164-6351, USAXiao-Lin Wu - Department of Animal Sciences, Washington State University, Pullman, WA 99164-6351, USAJennifer J Michal - Department of Animal Sciences, Washington State University, Pullman, WA 99164-6351, USAJerry J Reeves - Department of Animal Sciences, Washington State University, Pullman, WA 99164-6351, USAJan R Busboom - Department of Animal Sciences, Washington State University, Pullman, WA 99164-6351, USAGary H Thorgaard - School of Biological Sciences, Washington State University, Pullman, WA 99164-4236, USAZhihua Jiang - Department of Animal Sciences, Washington State University, Pullman, WA 99164-6351, USA
- Publication Details
- Journal of biotechnology, Vol.127(2), pp.177-187
- Academic Unit
- Animal Sciences, Department of; Biological Sciences, School of
- Publisher
- Elsevier B.V
- Identifiers
- 99900546655201842
- Language
- English
- Resource Type
- Journal article