Journal article
A single major chromosomal region controls natural killer cell-like activity in rainbow trout
Immunogenetics (New York), Vol.55(12), pp.825-835
03/2004
Handle:
https://hdl.handle.net/2376/101085
PMID: 14968267
Abstract
We report the identification of a single major chromosomal region controlling natural killer (NK) cell-like activity in rainbow trout (Oncorhynchus mykiss). A genetic map based on 484 AFLP and 39 microsatellite genotypes from 106 doubled haploid fish was constructed. These fish were produced by androgenesis from a hybrid of two clonal lines divergent in NK-like activity. NK-like activities for 75 of the doubled haploids were quantified by an in vitro chromium release assay utilizing 51Cr-labeled YAC-1 target cells. Composite interval mapping revealed a single major quantitative trait locus (QTL) associated with NK-like activity in this rainbow trout model. Genetic mapping revealed this QTL to also be unlinked to: fragmented MHC class I and MHC class II regions, the leukocyte receptor cluster, the natural killer cell enhancement factor (NKEF) gene, the RAG-1 gene, and two QTL associated with resistance to infectious pancreatic necrosis virus in rainbow trout. Collectively, these results extend the utility of rainbow trout as an immunological model and are consistent with the idea that a single chromosomal region homologous to the natural killer cell complex (NKC) located on syntenic portions of mouse chromosome (Chr) 6, human Chr 12, and rat Chr 4 may exist in a lower vertebrate model.
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Details
- Title
- A single major chromosomal region controls natural killer cell-like activity in rainbow trout
- Creators
- Anastasia Zimmerman - Department of Biology Massachusetts Institute of Technology Cambridge MA 02139 USAJason Evenhuis - Department of Animal Sciences Washington State University Pullman WA 99164-6332 USAGary Thorgaard - School of Biological Sciences and Center for Reproductive Biology Washington State University Pullman WA 99164-4236 USASandra Ristow - Agricultural Research Center Washington State University Pullman WA 99164-6240 USA
- Publication Details
- Immunogenetics (New York), Vol.55(12), pp.825-835
- Academic Unit
- Biological Sciences, School of
- Publisher
- Springer-Verlag; Berlin/Heidelberg
- Identifiers
- 99900546697001842
- Language
- English
- Resource Type
- Journal article