Journal article
A slot blot method for detection of ultraviolet photoproducts in DNA
Analytical biochemistry, Vol.229(2), pp.323-328
08/10/1995
Handle:
https://hdl.handle.net/2376/107647
PMID: 7485990
Abstract
A simple new method was established to detect the major uv photoproducts in DNA. A slot blot method, involving the use of T4 DNA polymerase-associated (3'--> 5') exonuclease digestion of uv-irradiated DNA, was used to detect (primarily) cyclobutane pyrimidine dimers and pyrimidine-pyrimidone (6-4) dimers. Hydrolysis of DNA by this enzyme is quantitatively blocked by both of these photoproducts. To detect (primarily) (6-4) dimers, Escherichia coli DNA photolyase was utilized to specifically reverse cyclobutane pyrimidine dimers.
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Details
- Title
- A slot blot method for detection of ultraviolet photoproducts in DNA
- Creators
- X Liu - Department of Biochemistry and Biophysics, Washington State University, Pullman 99164-4660, USAM J Smerdon
- Publication Details
- Analytical biochemistry, Vol.229(2), pp.323-328
- Academic Unit
- Molecular Biosciences, School of
- Publisher
- United States
- Grant note
- ES02614 / NIEHS NIH HHS
- Identifiers
- 99900547305501842
- Language
- English
- Resource Type
- Journal article