A soybean acyl carrier protein, GmACP, is important for root nodule symbiosis

Jun Wang; Katalin Tóth; Kiwamu Tanaka; Cuong T Nguyen; Zhe Yan; Laurent Brechenmacher; Jeremy Dahmen; Mingjie Chen; Jay J Thelen; Lijuan Qiu; Gary Stacey

doi:10.1094/MPMI-09-13-0269-R

Back

A soybean acyl carrier protein, GmACP, is important for root nodule symbiosis

Journal article

Open access

A soybean acyl carrier protein, GmACP, is important for root nodule symbiosis

Jun Wang, Katalin Tóth, Kiwamu Tanaka, Cuong T Nguyen, Zhe Yan, Laurent Brechenmacher, Jeremy Dahmen, Mingjie Chen, Jay J Thelen, Lijuan Qiu, …

Molecular plant-microbe interactions, Vol.27(5), pp.415-423

05/2014

DOI: https://doi.org/10.1094/MPMI-09-13-0269-R

Handle:

https://hdl.handle.net/2376/105663

PMID: 24400939

Files and links (1)

url

https://doi.org/10.1094/MPMI-09-13-0269-RView

Published (Version of record) Open

Abstract

Palmitic Acid - metabolism

Multigene Family

Molecular Sequence Data

Plant Roots - genetics

Phylogeny

Rhizobium - physiology

Soybeans - genetics

Stearic Acids - metabolism

Plant Roots - physiology

Plant Proteins - classification

Base Sequence

Plants, Genetically Modified

Gene Expression Regulation, Plant

Plant Proteins - metabolism

Acyl Carrier Protein - classification

Genes, Reporter

Nitrogen Fixation

Symbiosis

Amino Acid Sequence

Tobacco - metabolism

Acyl Carrier Protein - metabolism

Plant Roots - cytology

Plant Roots - microbiology

Sequence Analysis, DNA

Plant Proteins - genetics

Sequence Alignment

Plant Root Nodulation

Tobacco - genetics

Acyl Carrier Protein - genetics

Soybeans - microbiology

Soybeans - physiology

Soybeans - cytology

Legumes (members of family Fabaceae) establish a symbiotic relationship with nitrogen-fixing soil bacteria (rhizobia) to overcome nitrogen source limitation. Single root hair epidermal cells serve as the entry point for bacteria to infect the host root, leading to development of a new organ, the nodule, which the bacteria colonize. In the present study, the putative role of a soybean acyl carrier protein (ACP), GmACP (Glyma18g47950), was examined in nodulation. ACP represent an essential cofactor protein in fatty acid biosynthesis. Phylogenetic analysis of plant ACP protein sequences showed that GmACP was classified in a legume-specific clade. Quantitative reverse-transcription polymerase chain reaction analysis demonstrated that GmACP was expressed in all soybean tissues but showed higher transcript accumulation in nodule tissue. RNA interference-mediated gene silencing of GmACP resulted in a significant reduction in nodule numbers on soybean transgenic roots. Fluorescent protein-labeled GmACP was localized to plastids in planta, the site of de novo fatty acid biosynthesis in plants. Analysis of the fatty acid content of root tissue silenced for GmACP expression, as determined by gas chromatography-mass spectrometry, showed an approximately 22% reduction, specifically in palmitic and stearic acid. Taken together, our data provide evidence that GmACP plays an important role in nodulation.

Metrics

8 Record Views

Details

Title: A soybean acyl carrier protein, GmACP, is important for root nodule symbiosis
Creators: Jun Wang
Katalin Tóth
Kiwamu Tanaka
Cuong T Nguyen
Zhe Yan
Laurent Brechenmacher
Jeremy Dahmen
Mingjie Chen
Jay J Thelen
Lijuan Qiu
Gary Stacey
Publication Details: Molecular plant-microbe interactions, Vol.27(5), pp.415-423
Academic Unit: Plant Pathology, Department of
Publisher: United States
Identifiers: 99900546502101842
Language: English
Resource Type: Journal article