Journal article
A specific requirement for protein kinase C in activation of the respiratory burst oxidase of fertilization
The Journal of biological chemistry, Vol.265(14), pp.7717-7720
05/15/1990
Handle:
https://hdl.handle.net/2376/110088
PMID: 2335502
Abstract
Partially reduced oxygen species are toxic, yet activated sea urchin eggs produce H2O2, suggesting that the control of oxidant stress might be critical for early embryonic development. We show that the Ca2(+)-stimulated NADPH oxidase that generates H2O2 in the "respiratory burst" of fertilization is activated by a protein kinase, apparently to regulate the synthesis of this potentially lethal oxidant. The NADPH oxidase was separated into membrane and soluble fractions that were both required for H2O2 synthesis. The soluble fraction was further purified by anion exchange chromatography. The factor in the soluble fraction that activated the membrane-associated oxidase was demonstrated to be protein kinase C (PKC) by several criteria, including its Ca2+/phophatidylserine/diacyl-glycerol-stimulated histone kinase activity, its response to phorbol ester, its inhibition by a PKC pseudosubstrate peptide, and its replacement by purified mammalian PKC. Neither calmodulin-dependent kinase II, the catalytic subunit of cyclic AMP-dependent protein kinase, casein kinase II, nor myosin light chain kinase activated the oxidase. Although the PKC family has been ubiquitously implicated in cellular regulation, enzymes that require PKC for activation have not been identified; the respiratory burst oxidase is one such enzyme.
Metrics
7 Record Views
Details
- Title
- A specific requirement for protein kinase C in activation of the respiratory burst oxidase of fertilization
- Creators
- J W Heinecke - Department of Medicine, University of Washington, Seattle 98195K E MeierJ A LorenzenB M Shapiro
- Publication Details
- The Journal of biological chemistry, Vol.265(14), pp.7717-7720
- Academic Unit
- Pharmaceutical Sciences, Department of
- Publisher
- United States
- Identifiers
- 99900547496201842
- Language
- English
- Resource Type
- Journal article