Journal article
A survey of SL1-spliced transcripts from the root-lesion nematode Pratylenchus penetrans
Molecular genetics and genomics : MGG, Vol.272(2), pp.138-148
09/2004
Handle:
https://hdl.handle.net/2376/113263
PMID: 15338281
Abstract
Plant-parasitic nematodes are important and cosmopolitan pathogens of crops. Here, we describe the generation and analysis of 1928 expressed sequence tags (ESTs) of a splice-leader 1 (SL1) library from mixed life stages of the root-lesion nematode Pratylenchus penetrans. The ESTs were grouped into 420 clusters and classified by function using the Gene Ontology (GO) hierarchy and the Kyoto KEGG database. Approximately 80% of all translated clusters show homology to Caenorhabditis elegans proteins, and 37% of the C. elegans gene homologs had confirmed phenotypes as assessed by RNA interference tests. Use of an SL1-PCR approach, while ensuring the cloning of the 5′ ends of mRNAs, has demonstrated bias toward short transcripts. Putative nematode-specific and Pratylenchus -specific genes were identified, and their implications for nematode control strategies are discussed.
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Details
- Title
- A survey of SL1-spliced transcripts from the root-lesion nematode Pratylenchus penetrans
- Creators
- M Mitreva - Genome Sequencing Center, Department of GeneticsWashington University School of MedicineSt. LouisMO 63108USAA Elling - Department of Plant PathologyIowa State UniversityAmesIA 50011USAM Dante - Genome Sequencing Center, Department of GeneticsWashington University School of MedicineSt. LouisMO 63108USAA Kloek - Divergence Inc.893 North Warson RoadSt. LouisMO 63141USAA Kalyanaraman - Department of Electrical and Computer EngineeringIowa State UniversityAmesIA 50011USAS Aluru - Department of Electrical and Computer EngineeringIowa State UniversityAmesIA 50011USAS Clifton - Genome Sequencing Center, Department of GeneticsWashington University School of MedicineSt. LouisMO 63108USAD Bird - The Center for the Biology of Nematode ParasitismNorth Carolina State UniversityRaleighNC 27695USAT Baum - Department of Plant PathologyIowa State UniversityAmesIA 50011USAJ McCarter - Divergence Inc.893 North Warson RoadSt. LouisMO 63141USA
- Publication Details
- Molecular genetics and genomics : MGG, Vol.272(2), pp.138-148
- Academic Unit
- Electrical Engineering and Computer Science, School of
- Publisher
- Springer-Verlag; Berlin/Heidelberg
- Identifiers
- 99900548039501842
- Language
- English
- Resource Type
- Journal article