A systematic analysis of a deep mouse epididymal sperm proteome

Theodore Chauvin; Fang Xie; Tao Liu; Carrie D Nicora; Feng Yang; David G Camp, 2nd; Richard D Smith; Kenneth P Roberts

doi:10.1095/biolreprod.112.104208

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A systematic analysis of a deep mouse epididymal sperm proteome

Journal article

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Peer reviewed

A systematic analysis of a deep mouse epididymal sperm proteome

Theodore Chauvin, Fang Xie, Tao Liu, Carrie D Nicora, Feng Yang, David G Camp, 2nd, Richard D Smith and Kenneth P Roberts

Biology of reproduction, Vol.87(6), pp.141-141

06/2012

DOI: https://doi.org/10.1095/biolreprod.112.104208

Handle:

https://hdl.handle.net/2376/107581

PMID: 23115268

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Abstract

Up-Regulation

Peptide Fragments - metabolism

Signal Transduction

Testis - cytology

Down-Regulation

Infertility, Male - etiology

Transcriptome

Male

Mutant Proteins - metabolism

Epididymis - cytology

Chromatography, High Pressure Liquid

Spermatozoa - metabolism

Proteome - chemistry

Tandem Mass Spectrometry

Peptide Fragments - chemistry

Animals

Databases, Protein

Infertility, Male - metabolism

Mice

Proteomics - methods

Proteome - metabolism

Cluster Analysis

Spermatozoa are highly specialized cells that, when mature, are capable of navigating the female reproductive tract and fertilizing an oocyte. The sperm cell is thought to be largely quiescent in terms of transcriptional and translational activity. As a result, once it has left the male reproductive tract, the sperm cell is essentially operating with a static population of proteins. It therefore is theoretically possible to understand the protein networks contained in a sperm cell and to deduce its cellular function capabilities. To this end, we performed a proteomic analysis of mouse sperm isolated from the cauda epididymis and confidently identified 2850 proteins, which to our knowledge is the most comprehensive sperm proteome for any species reported to date. These proteins comprise many complete cellular pathways, including those for energy production via glycolysis, beta-oxidation and oxidative phosphorylation, protein folding and transport, and cell signaling systems. This proteome should prove a useful tool for assembly and testing of protein networks important for sperm function.

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Title: A systematic analysis of a deep mouse epididymal sperm proteome
Creators: Theodore Chauvin - School of Molecular Biosciences, Washington State University, Spokane, Washington 99201, USA
Fang Xie
Tao Liu
Carrie D Nicora
Feng Yang
David G Camp, 2nd
Richard D Smith
Kenneth P Roberts
Publication Details: Biology of reproduction, Vol.87(6), pp.141-141
Academic Unit: Elson S. Floyd College of Medicine; Biomedical Sciences, Department of
Publisher: United States
Grant note: P41 GM103493 / NIGMS NIH HHS P41 RR018522 / NCRR NIH HHS 8 P41 GM103493-10 / NIGMS NIH HHS 5 P41 RR018522-10 / NCRR NIH HHS
Identifiers: 99900547204501842
Language: English
Resource Type: Journal article