A unique phospholipid organization in bovine erythrocyte membranes

J Florin-Christensen; C. E Suarez; M Florin-Christensen; M Wainszelbaum; W. C Brown; T. F McElwain; G. H Palmer

doi:10.1073/pnas.131580998

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A unique phospholipid organization in bovine erythrocyte membranes

Journal article

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A unique phospholipid organization in bovine erythrocyte membranes

J Florin-Christensen, C. E Suarez, M Florin-Christensen, M Wainszelbaum, W. C Brown, T. F McElwain and G. H Palmer

Proceedings of the National Academy of Sciences - PNAS, Vol.98(14), pp.7736-7741

07/03/2001

DOI: https://doi.org/10.1073/pnas.131580998

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https://hdl.handle.net/2376/102828

PMCID: PMC35411

PMID: 11427712

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Abstract

Biological Sciences

Ruminant erythrocytes are remarkable for their choline-phospholipid anomalies; namely, low or absent phosphatidylcholine (PC) along with high sphingomyelin levels. Here, we report another anomaly in bovine erythrocytes that affects aminophospholipids: phosphatidylethanolamine (PE) shows an extreme asymmetry, with only 2% of the total present in the outer leaflet. Furthermore, we found that phospholipase A 2 , an enzyme located on the external surface of the erythrocytes, shows higher activity against PC than against PE. In addition, we observed that acylation of PE is by far the most important biosynthetic event in this system. We propose that deacylation of PE and PC by phospholipase A 2 to generate lysocompounds, followed by selective reacylation of lyso-PE in the inner leaflet, can account for the compositional and architectural peculiarities of bovine erythrocyte membranes.

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Title: A unique phospholipid organization in bovine erythrocyte membranes
Creators: J Florin-Christensen - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164-7040
C. E Suarez - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164-7040
M Florin-Christensen - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164-7040
M Wainszelbaum - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164-7040
W. C Brown - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164-7040
T. F McElwain - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164-7040
G. H Palmer - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164-7040
Publication Details: Proceedings of the National Academy of Sciences - PNAS, Vol.98(14), pp.7736-7741
Academic Unit: Veterinary Microbiology and Pathology, Department of; Paul G. Allen School for Global Animal Health
Publisher: The National Academy of Sciences
Identifiers: 99900546801701842
Language: English
Resource Type: Journal article