Journal article
A universal lateral flow biosensor for proteins and DNAs based on the conformational change of hairpin oligonucleotide and its use for logic gate operations
Biosensors & bioelectronics, Vol.61, pp.598-604
11/15/2014
Handle:
https://hdl.handle.net/2376/113744
PMID: 24967748
Abstract
A universal lateral flow biosensor for proteins and DNAs was designed on the base of target-induced conformational changes of hairpin oligonucleotide (HO). CEA (carcinoembryonic antigen) protein and c-DNA were detected both with the naked eye and a strip reader. The scheme of detecting proteins and DNAs were based on the unique molecular recognition properties of HO to the targets to form different quantities of “active” biotin groups on the surface of gold nanoparticles (AuNPs). The output of the strip is the color of the test line, which inspired us to combine strip biosensor with logic gate. Two strip logic gates (“OR” and “INH”) were designed in our paper and the combinatorial logic gates in our paper could be used to make high-throughput judgment about what targets were present in the input samples according to the output results. The biosensor facilitates a portable analysis at ambient temperature as it is simple to be conducted and no requirement of training is needed. The strip logic system is proved an excellent selection and can operate effectively as well as in human serum samples. Therefore, we indicate that such logic strips a foreseeable promise in application of intelligent point-of-care and in-field diagnostics.
Schematic illustration of the design strategy of the strip “OR” logic gate.▪
•A pair of strip logic gates (“OR” and “INHIBIT”) for proteins and DNAs is designed.•The detecting scheme is based on molecular recognition of hairpin oligonucleotides.•The biosensor facilitates a portable analysis at ambient temperature.•The combinatorial logic gates could be used to make high-throughput judgment.
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Details
- Title
- A universal lateral flow biosensor for proteins and DNAs based on the conformational change of hairpin oligonucleotide and its use for logic gate operations
- Creators
- Yan Huang - Hubei UniversityWei Wen - Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules & College of Chemistry and Chemical Engineering, Hubei University, Wuhan 430062, PR ChinaDan Du - School of Mechanical and Materials Engineering, Washington State University, Pullman 99164, USAXiuhua Zhang - Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules & College of Chemistry and Chemical Engineering, Hubei University, Wuhan 430062, PR ChinaShengfu Wang - Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules & College of Chemistry and Chemical Engineering, Hubei University, Wuhan 430062, PR ChinaYuehe Lin - School of Mechanical and Materials Engineering, Washington State University, Pullman 99164, USA
- Publication Details
- Biosensors & bioelectronics, Vol.61, pp.598-604
- Academic Unit
- School of Mechanical and Materials Engineering
- Publisher
- Elsevier B.V
- Number of pages
- 7
- Identifiers
- 99900547328301842
- Language
- English
- Resource Type
- Journal article