A β-barrel outer membrane protein facilitates cellular uptake of polychlorophenols in Cupriavidus necator

Sara Mae Belchik; Scott M Schaeffer; Shelley Hasenoehrl; Luying Xun

doi:10.1007/s10532-009-9313-8

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A β-barrel outer membrane protein facilitates cellular uptake of polychlorophenols in Cupriavidus necator

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A β-barrel outer membrane protein facilitates cellular uptake of polychlorophenols in Cupriavidus necator

Sara Mae Belchik, Scott M Schaeffer, Shelley Hasenoehrl and Luying Xun

Biodegradation (Dordrecht), Vol.21(3), pp.431-439

06/2010

DOI: https://doi.org/10.1007/s10532-009-9313-8

Handle:

https://hdl.handle.net/2376/105844

PMCID: PMC2908400

PMID: 19937267

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https://doi.org/10.1007/s10532-009-9313-8View

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Abstract

Outer membrane transporter

Trichlorophenol biodegradation

β-Barrel protein

Substrate uptake

Gram negative bacteria

The tcpRXABCYD operon of Cupriavidus necator JMP134 is involved in the degradation of 2,4,6-trichlorophenol (TCP). All of the gene products except TcpY have assigned functions in TCP metabolism. Sequence comparison identified TcpY as a member of COG4313, a group of hypothetical proteins. TcpY has a signal peptide, indicating it is a membrane or secreted protein. Secondary structure and topology analysis indicated TcpY as a β -barrel outer membrane protein, similar to the Escherichia coli outer membrane protein FadL that transports hydrophobic long-chain fatty acids. Constitutive expression of tcpY in two C. necator strains rendered the cells more sensitive to TCP and other polychlorophenols. Further, C. necator JMP134 expressing cloned tcpY transported more TCP into the cell than a control with the cloning vector. Thus, TcpY is an outer membrane protein that facilitates the passing of polychlorophenols across the outer membrane of C. necator . Similarly, other COG4313 proteins are possibly outer membrane transporters of hydrophobic aromatic compounds.

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Title: A β-barrel outer membrane protein facilitates cellular uptake of polychlorophenols in Cupriavidus necator
Creators: Sara Mae Belchik - School of Molecular Biosciences, Washington State University, Life Sciences Building, Room 202, 100 Dairy Road, Pullman, WA 99164-7520, USA
Scott M Schaeffer - School of Molecular Biosciences, Washington State University, Life Sciences Building, Room 202, 100 Dairy Road, Pullman, WA 99164-7520, USA
Shelley Hasenoehrl - School of Molecular Biosciences, Washington State University, Life Sciences Building, Room 202, 100 Dairy Road, Pullman, WA 99164-7520, USA
Luying Xun - School of Molecular Biosciences, Washington State University, Life Sciences Building, Room 202, 100 Dairy Road, Pullman, WA 99164-7520, USA
Publication Details: Biodegradation (Dordrecht), Vol.21(3), pp.431-439
Academic Unit: Molecular Biosciences, School of
Identifiers: 99900547087501842
Language: English
Resource Type: Journal article