Journal article
AMP-activated protein kinase stimulates Warburg-like glycolysis and activation of satellite cells during muscle regeneration
The Journal of biological chemistry, Vol.290(44), pp.26445-26456
10/30/2015
Handle:
https://hdl.handle.net/2376/107064
PMCID: PMC4646303
PMID: 26370082
Abstract
Satellite cells are the major myogenic stem cells residing inside skeletal muscle and are indispensable for muscle regeneration. Satellite cells remain largely quiescent but are rapidly activated in response to muscle injury, and the derived myogenic cells then fuse to repair damaged muscle fibers or form new muscle fibers. However, mechanisms eliciting metabolic activation, an inseparable step for satellite cell activation following muscle injury, have not been defined. We found that a noncanonical Sonic Hedgehog (Shh) pathway is rapidly activated in response to muscle injury, which activates AMPK and induces a Warburg-like glycolysis in satellite cells. AMPKα1 is the dominant AMPKα isoform expressed in satellite cells, and AMPKα1 deficiency in satellite cells impairs their activation and myogenic differentiation during muscle regeneration. Drugs activating noncanonical Shh promote proliferation of satellite cells, which is abolished because of satellite cell-specific AMPKα1 knock-out. Taken together, AMPKα1 is a critical mediator linking noncanonical Shh pathway to Warburg-like glycolysis in satellite cells, which is required for satellite activation and muscle regeneration.
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Details
- Title
- AMP-activated protein kinase stimulates Warburg-like glycolysis and activation of satellite cells during muscle regeneration
- Creators
- Xing Fu - From the Department of Animal Sciences and Department of Pharmaceutical Sciences, Washington Center for Muscle Biology andMei-Jun Zhu - the School of Food Sciences, Washington State University, Pullman, Washington 99164Mike V Dodson - From the Department of Animal Sciences and Department of Pharmaceutical Sciences, Washington Center for Muscle Biology andMin Du - From the Department of Animal Sciences and Department of Pharmaceutical Sciences, Washington Center for Muscle Biology and min.du@wsu.edu
- Publication Details
- The Journal of biological chemistry, Vol.290(44), pp.26445-26456
- Academic Unit
- Animal Sciences, Department of
- Publisher
- United States
- Grant note
- R01 HD067449 / NICHD NIH HHS 1R01HD067449 / NICHD NIH HHS
- Identifiers
- 99900546899101842
- Language
- English
- Resource Type
- Journal article