AXR1-ECR1–Dependent Conjugation of RUB1 to the Arabidopsis Cullin AtCUL1 Is Required for Auxin Response

Juan C del Pozo; Sunethra Dharmasiri; Hanjo Hellmann; Loni Walker; William M Gray; Mark Estelle

doi:10.1105/tpc.010282

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AXR1-ECR1–Dependent Conjugation of RUB1 to the Arabidopsis Cullin AtCUL1 Is Required for Auxin Response

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AXR1-ECR1–Dependent Conjugation of RUB1 to the Arabidopsis Cullin AtCUL1 Is Required for Auxin Response

Juan C del Pozo, Sunethra Dharmasiri, Hanjo Hellmann, Loni Walker, William M Gray and Mark Estelle

The Plant cell, Vol.14(2), pp.421-433

02/2002

DOI: https://doi.org/10.1105/tpc.010282

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https://hdl.handle.net/2376/105217

PMCID: PMC152922

PMID: 11884684

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Abstract

Mutations in the AXR1 gene result in a reduction in auxin response and diverse defects in auxin-regulated growth and development. In a previous study, we showed that AXR1 forms a heterodimer with the ECR1 protein. This enzyme activates the ubiquitin-related protein RUB1 in vitro. Furthermore, we showed that the Skp1-Cul1/Cdc53-F-box (SCF) subunit AtCUL1 is modified by RUB1 in vivo. In this report, we demonstrate that the formation of RUB-AtCUL1 is dependent on AXR1 and ECR1 in vivo. The expression of AXR1 and ECR1 is restricted to zones of active cell division and cell elongation, consistent with their role in growth regulation. These results provide strong support for a model in which RUB conjugation of AtCUL1 affects the function of SCF E3s that are required for auxin response.

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Title: AXR1-ECR1–Dependent Conjugation of RUB1 to the Arabidopsis Cullin AtCUL1 Is Required for Auxin Response
Creators: Juan C del Pozo - Molecular Cell and Developmental Biology, Institute for Cellular and Molecular Biology, University of Texas at Austin, Texas 78712
Sunethra Dharmasiri - Molecular Cell and Developmental Biology, Institute for Cellular and Molecular Biology, University of Texas at Austin, Texas 78712
Hanjo Hellmann - Molecular Cell and Developmental Biology, Institute for Cellular and Molecular Biology, University of Texas at Austin, Texas 78712
Loni Walker - Molecular Cell and Developmental Biology, Institute for Cellular and Molecular Biology, University of Texas at Austin, Texas 78712
William M Gray - Molecular Cell and Developmental Biology, Institute for Cellular and Molecular Biology, University of Texas at Austin, Texas 78712
Mark Estelle - Molecular Cell and Developmental Biology, Institute for Cellular and Molecular Biology, University of Texas at Austin, Texas 78712
Publication Details: The Plant cell, Vol.14(2), pp.421-433
Academic Unit: Biological Sciences, School of
Publisher: American Society of Plant Biologists
Identifiers: 99900546769001842
Language: English
Resource Type: Journal article