Journal article
Absence of Spontaneous Central Nervous System Remyelination in Class II-deficient Mice Infected with Theiler’s Virus
Journal of neuropathology and experimental neurology, Vol.58(1), pp.78-91
01/1999
Handle:
https://hdl.handle.net/2376/109954
PMCID: PMC5444470
PMID: 10068316
Abstract
We previously showed that Theiler’s murine encephalomyelitis virus (TMEV)-infected major histocompatibility complex (MHC) class II-deficient mice develop both demyelination and neurologic deficits, whereas MHC class I-deficient mice develop demyelination but no neurologic deficits. The absence of neurologic deficits in the class I-deficient mice was associated with preserved sodium channel densities in demyelinated lesions, a relative preservation of axons, and extensive spontaneous remyelination. In this study, we investigated whether TMEV-infected class II-deficient mice, which have an identical genetic background (C57BL/6 × 129) as the class I-deficient mice, have preserved axons and spontaneous myelin repair following chronic TMEV-infection. Both class I- and class II-deficient mice showed similar extents of demyelination of the spinal cord white matter 4 months after TMEV infection. However, the class I-deficient mice demonstrated remyelination by oligodendrocytes, whereas class II-deficient mice showed minimal if any myelin repair. Demyelinated lesions, characterized by inflammatory infiltrates in both mutants, revealed disruption of axons in class II- but not class I-deficient mice. Further characterization revealed that even though class II-deficient mice lacked TMEV-specific IgG, they had virus-specific IgM, which, however, did not neutralize TMEV in vitro. In addition, class II-deficient mice developed TMEV-specific cytotoxic T-lymphocytes in the CNS during the acute (7 days) disease, but these cytotoxic lymphocytes were not present in the chronic stage of disease, despite a high titer of infectious virus throughout the disease. We envision that the presence of demyelination, high virus titer, absence of remyelination, and axonal disruption in chronically infected class II-deficient mice contributes to the development of paralytic disease.
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Details
- Title
- Absence of Spontaneous Central Nervous System Remyelination in Class II-deficient Mice Infected with Theiler’s Virus
- Creators
- M. Kariuki NjengaPaul D MurrayDorian McGavernXiaoqi LinKristen M DrescherMoses Rodriguez
- Publication Details
- Journal of neuropathology and experimental neurology, Vol.58(1), pp.78-91
- Academic Unit
- Paul G. Allen School for Global Animal Health
- Identifiers
- 99900546922001842
- Language
- English
- Resource Type
- Journal article