Journal article
Acceptance of skin grafts by isogenic rainbow trout (Oncorhynchus mykiss)
American journal of veterinary research, Vol.57(11), pp.1576-1579
11/1996
Handle:
https://hdl.handle.net/2376/107646
PMID: 8915432
Abstract
To test the immunocompetence of isogenic families of rainbow trout by measuring their ability to accept or reject skin grafts.
3 families of isogenic rainbow trout (Oncorhynchus mykiss), produced by mating homozygous females and homozygous males, plus 4 chinook salmon (O tshawytscha) were used in these experiments.
Grafts (allografts, members of the same family; autografts, donor and recipient were the same fish; and xenografts, O tshawytscha as donor) were exchanged. Grafts were applied on day 0 and removed on day 21, placed in neutral-buffered formalin, and embedded in paraffin. Lymphocytes and nuclei were counted in representative stained sections in the epidermis, dermis, and hypodermis. Results were analyzed by univariate analysis, using the Shapiro-Wilk statistic.
Autografts were retained and minimal histologic changes were apparent. Allografts were histologically similar to autografts. Xenografts were rejected.
Results indicate that the immune system of isogenic rainbow trout is unable to distinguish between family members within isogenic families, but that a vigorous response is mounted against chinook salmon xenografts. The isogenic rainbow trout are immunocompetent with respect to the phenomenon of graft rejection.
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Details
- Title
- Acceptance of skin grafts by isogenic rainbow trout (Oncorhynchus mykiss)
- Creators
- S S Ristow - Department of Animal Sciences, Washington State University, Pullman 99164-6332, USAJ M de AvilaT J BaldwinP A WheelerG H Thorgaard
- Publication Details
- American journal of veterinary research, Vol.57(11), pp.1576-1579
- Academic Unit
- Veterinary Microbiology and Pathology, Department of; Biological Sciences, School of
- Publisher
- United States
- Grant note
- 1-R01 RR06654 / NCRR NIH HHS O1 ES04766 / NIEHS NIH HHS
- Identifiers
- 99900547142701842
- Language
- English
- Resource Type
- Journal article