Accessing DNA damage in chromatin: Preparing the chromatin landscape for base excision repair

Yesenia Rodriguez; John M Hinz; Michael J Smerdon

doi:10.1016/j.dnarep.2015.04.021

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Accessing DNA damage in chromatin: Preparing the chromatin landscape for base excision repair

Journal article

Open access

Peer reviewed

Accessing DNA damage in chromatin: Preparing the chromatin landscape for base excision repair

Yesenia Rodriguez, John M Hinz and Michael J Smerdon

DNA repair, Vol.32, pp.113-119

08/2015

DOI: https://doi.org/10.1016/j.dnarep.2015.04.021

Handle:

https://hdl.handle.net/2376/115226

PMCID: PMC4522338

PMID: 25957487

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https://doi.org/10.1016/j.dnarep.2015.04.021View

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Abstract

Uracil-DNA Glycosidase - metabolism

Nucleosomes - chemistry

Humans

Chromatin Assembly and Disassembly

Nucleosomes - metabolism

DNA - metabolism

Histones - genetics

Adenosine Triphosphate - metabolism

DNA Repair

Uracil - metabolism

Protein Processing, Post-Translational

DNA Damage

DNA Polymerase beta - metabolism

Histones - metabolism

DNA Polymerase beta - genetics

Uracil-DNA Glycosidase - genetics

DNA damage in chromatin comes in many forms, including single base lesions that induce base excision repair (BER). We and others have shown that the structural location of DNA lesions within nucleosomes greatly influences their accessibility to repair enzymes. Indeed, a difference in the location of uracil as small as one-half turn of the DNA backbone on the histone surface can result in a 10-fold difference in the time course of its removal in vitro. In addition, the cell has evolved several interdependent processes capable of enhancing the accessibility of excision repair enzymes to DNA lesions in nucleosomes, including post-translational modification of histones, ATP-dependent chromatin remodeling and interchange of histone variants in nucleosomes. In this review, we focus on different factors that affect accessibility of BER enzymes to nucleosomal DNA.

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Title: Accessing DNA damage in chromatin: Preparing the chromatin landscape for base excision repair
Creators: Yesenia Rodriguez - School of Molecular Biosciences, Washington State University, Pullman, WA 99164-7520, United States
John M Hinz - School of Molecular Biosciences, Washington State University, Pullman, WA 99164-7520, United States
Michael J Smerdon - School of Molecular Biosciences, Washington State University, Pullman, WA 99164-7520, United States. Electronic address: smerdon@vetmed.wsu.edu
Publication Details: DNA repair, Vol.32, pp.113-119
Academic Unit: Molecular Biosciences, School of
Publisher: Netherlands
Grant note: R37 ES002614 / NIEHS NIH HHS R01 ES002614 / NIEHS NIH HHS ES002614 / NIEHS NIH HHS T32 GM008336 / NIGMS NIH HHS R01 ES004106 / NIEHS NIH HHS ES004106 / NIEHS NIH HHS R21 ES020955 / NIEHS NIH HHS ES020955 / NIEHS NIH HHS
Identifiers: 99900547569701842
Language: English
Resource Type: Journal article