Actions of anti-Mullerian hormone on the ovarian transcriptome to inhibit primordial to primary follicle transition

Eric Nilsson; Natalie Rogers; Michael K Skinner

doi:10.1530/REP-07-0119

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Actions of anti-Mullerian hormone on the ovarian transcriptome to inhibit primordial to primary follicle transition

Journal article

Open access

Peer reviewed

Actions of anti-Mullerian hormone on the ovarian transcriptome to inhibit primordial to primary follicle transition

Eric Nilsson, Natalie Rogers and Michael K Skinner

Reproduction (Cambridge, England), Vol.134(2), pp.209-221

08/2007

DOI: https://doi.org/10.1530/REP-07-0119

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https://hdl.handle.net/2376/104144

PMCID: PMC8260025

PMID: 17660231

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Abstract

Fibroblast Growth Factor 7 - pharmacology

Transcription, Genetic - drug effects

Oligonucleotide Array Sequence Analysis

Anti-Mullerian Hormone - pharmacology

Fibroblast Growth Factor 2 - pharmacology

MAP Kinase Signaling System

Ovarian Follicle - drug effects

Stem Cell Factor - pharmacology

Ovarian Follicle - physiology

Ovary - drug effects

Organ Culture Techniques

Ovary - metabolism

Animals, Newborn

Primary Ovarian Insufficiency - metabolism

Gene Expression Regulation, Developmental - drug effects

Gene Expression Profiling - methods

Recombinant Proteins - pharmacology

Rats, Sprague-Dawley

Oocytes - physiology

Transforming Growth Factor beta - metabolism

Gene Expression

Signal Transduction

The oocytes found within the primordial follicles of mammalian ovaries remain quiescent for months to years until they receive the appropriate signals to undergo the primordial to primary follicle transition and initiate folliculogenesis. The molecular mechanisms and extracellular signaling factors that regulate this process remain to be fully elucidated. The current study investigates the mechanisms utilized by anti-Müllerian hormone (AMH; i.e. Müllerian inhibitory substance) to inhibit the primordial to primary follicle transition. Ovaries from 4-day-old rats were placed into organ culture and incubated in the absence or presence of AMH, either alone or in combination with known stimulators of follicle transition, including basic fibroblast growth factor (bFGF), kit ligand (KITL), or keratinocyte growth factor (KGF). Following 10 days of culture, the ovaries were sectioned, stained, and morphologically evaluated to determine the percentage of primordial versus developing follicles. As previously demonstrated, AMH treatment decreased primordial to primary follicle transition. Interestingly, AMH inhibited the stimulatory actions of KITL, bFGF, and KGF. Therefore, AMH can inhibit the basal and stimulated development of primordial follicles. To investigate the mechanism of AMH actions, the influence AMH has on the ovarian transcriptome was analyzed. AMH treatment when compared with controls was found to alter the expression of 707 genes. The overall effect of AMH exposure is to decrease the expression of stimulatory factors, increase the expression of inhibitory factors, and regulate cellular pathways (e.g. transforming growth factor beta signaling pathway) that result in the inhibition of primordial follicle development. Analysis of the regulatory factors and cellular pathways altered by AMH provides a better understanding of the molecular control of primordial follicle development.

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Title: Actions of anti-Mullerian hormone on the ovarian transcriptome to inhibit primordial to primary follicle transition
Creators: Eric Nilsson - Center for Reproductive Biology, School of Molecular Biosciences, Washington State University, Pullman, Washington 99164-4231, USA
Natalie Rogers
Michael K Skinner
Publication Details: Reproduction (Cambridge, England), Vol.134(2), pp.209-221
Academic Unit: Biological Sciences, School of
Publisher: England
Identifiers: 99900546745001842
Language: English
Resource Type: Journal article