Adenovirus-mediated interference of FABP4 regulates mRNA expression of ADIPOQ, LEP and LEPR in bovine adipocytes

S Wei; L S Zan; H B Wang; G Cheng; M Du; Z Jiang; G J Hausman; D C McFarland; M V Dodson

doi:10.4238/2013.January.4.21

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Adenovirus-mediated interference of FABP4 regulates mRNA expression of ADIPOQ, LEP and LEPR in bovine adipocytes

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Adenovirus-mediated interference of FABP4 regulates mRNA expression of ADIPOQ, LEP and LEPR in bovine adipocytes

S Wei, L S Zan, H B Wang, G Cheng, M Du, Z Jiang, G J Hausman, D C McFarland and M V Dodson

Genetics and molecular research, Vol.12(1), pp.494-505

02/27/2013

DOI: https://doi.org/10.4238/2013.January.4.21

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https://hdl.handle.net/2376/100829

PMID: 23315880

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Abstract

RNA, Small Interfering - genetics

Receptors, Leptin - genetics

Gene Expression - genetics

Leptin - metabolism

Fatty Acid-Binding Proteins - metabolism

PPAR gamma - metabolism

Cell Differentiation - genetics

Cattle

Adiponectin - genetics

Adiponectin - metabolism

Lipid Metabolism - genetics

CCAAT-Enhancer-Binding Proteins - genetics

Sterol Regulatory Element Binding Protein 1 - metabolism

PPAR gamma - genetics

CCAAT-Enhancer-Binding Proteins - metabolism

Cell Line

RNA, Messenger - genetics

Leptin - genetics

Fatty Acid-Binding Proteins - genetics

Genetic Vectors - genetics

Animals

Sterol Regulatory Element Binding Protein 1 - genetics

Adipocytes - metabolism

Adenoviridae - metabolism

Receptors, Leptin - metabolism

Fatty acid binding protein 4 (FABP4) is an important adipocyte gene, with roles in fatty acid transport and fat deposition in animals as well as human metabolic syndrome. However, little is known about the functional regulation of FABP4 at the cellular level in bovine. We designed and selected an effective shRNA (small hairpin RNA) against bovine FABP4, constructed a corresponding adenovirus (AD-FABP4), and then detected its influence on mRNA expression of four differentiation-related genes (PPAR(y), CEBPA, CEBPB, and SREBF1) and three lipid metabolism-related genes (ADIPOQ, LEP and LEPR) of adipocytes. The FABP4 mRNA content, derived from bovine adipocytes, decreased by 41% (P < 0.01) after 24 h and 66% (P < 0.01) after 72 h of AD-FABP4 infection. However, lower mRNA content of FABP4 did not significantly alter levels of differentiation-related gene expression at 24 h following AD-FABP4 treatment of bovine-derived preadipocytes (P = 0.54, 0.78, 0.89, and 0.94, respectively). Meanwhile, knocking down (partially silencing) FABP4 significantly decreased ADIPOQ (P < 0.05) and LEP (P < 0.01) gene expression after 24 h of AD-FABP4 treatment, decreased ADIPOQ (P < 0.01) and LEP (P < 0.01) gene expression, but increased LEPR mRNA expression (P < 0.01) after a 72-h treatment of bovine preadipocytes. We conclude that FABP4 plays a role in fat deposition and metabolic syndrome by regulating lipid metabolism-related genes (such as ADIPOQ, LEP and LEPR), without affecting the ability of preadipocytes to differentiate into adipocytes.

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Title: Adenovirus-mediated interference of FABP4 regulates mRNA expression of ADIPOQ, LEP and LEPR in bovine adipocytes
Creators: S Wei - College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, P.R. China
L S Zan
H B Wang
G Cheng
M Du
Z Jiang
G J Hausman
D C McFarland
M V Dodson
Publication Details: Genetics and molecular research, Vol.12(1), pp.494-505
Academic Unit: Animal Sciences, Department of
Publisher: Brazil
Identifiers: 99900546553701842
Language: English
Resource Type: Journal article