Journal article
Advances in the diagnosis of some parasitic diseases by monoclonal antibody-based enzyme-linked immunosorbent assays
Revue scientifique et technique (International Office of Epizootics), Vol.12(2), pp.425-433
06/1993
Handle:
https://hdl.handle.net/2376/106846
PMID: 8400383
Abstract
Advances in diagnostic assays for parasitic diseases include the use of monoclonal antibodies (MAbs) in antigen capture and competitive inhibition enzyme-linked immunosorbent assays (C-ELISA). Antigen capture ELISAs for Anaplasma marginale and Cryptosporidium parvum provide direct detection of these parasites during clinical disease, and the C-ELISA format has been adapted for detection of anti-Babesia equi, anti-A. marginale and anti-bluetongue virus antibodies. False-positive results may occur when antigen preparations in other ELISA formats are contaminated with Escherichia coli, erythrocyte or cell-culture antigens. The C-ELISA format overcomes problems of antigen purity, since the specificity of the C-ELISA depends solely on the MAb used. For this reason, the C-ELISA format is highly suited for use with recombinant antigens. Also, the use of recombinant protein in diagnostic assays precludes the need to infect animals for antigen production when the antigen cannot be produced in cell culture.
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Details
- Title
- Advances in the diagnosis of some parasitic diseases by monoclonal antibody-based enzyme-linked immunosorbent assays
- Creators
- D P Knowles, Jr - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164-7030J R Gorham
- Publication Details
- Revue scientifique et technique (International Office of Epizootics), Vol.12(2), pp.425-433
- Publisher
- France
- Identifiers
- 99900547099301842
- Language
- English
- Resource Type
- Journal article