Journal article
Alterations in GHRH binding and GHRH receptor mRNA in the pituitary of adult dw/dw rats
Peptides (New York, N.Y. : 1980), Vol.23(8), pp.1497-1502
2002
Handle:
https://hdl.handle.net/2376/110232
PMID: 12182953
Abstract
Lewis dwarf (
dw/
dw) rats exhibit growth hormone (GH) deficiency and growth retardation linked to a malfunction of GHRH signaling. In this study, GHRH-receptor (GHRH-R) binding and mRNA in the pituitary of adult male
dw/
dw and age-matched normal Lewis rats was measured by radioligand binding assay and real-time PCR. Only one of nine pools of
dw/
dw pituitary membranes revealed detectable binding of [His
1,
125
I
-Tyr
10, Nle
27]hGHRH(1–32) amide (
B
max; 4.3
fmol/mg protein). In contrast, GHRH-R binding was 22.4±2.60
fmol/mg protein in normal Lewis rats. mRNA for GHRH-R was detectable in all
dw/
dw rat pituitaries examined, averaging 21% that of Lewis rats. Low expression of GHRH-R reflects reduced GHRH-R mRNA as well as a possible reduction in translation of the receptor protein.
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Details
- Title
- Alterations in GHRH binding and GHRH receptor mRNA in the pituitary of adult dw/dw rats
- Creators
- János Gardi - Department of Veterinary and Comparative Anatomy, Pharmacology and Physiology, Washington State University, Pullman, WA 99164-6520, USARobert C Speth - Department of Veterinary and Comparative Anatomy, Pharmacology and Physiology, Washington State University, Pullman, WA 99164-6520, USAPing Taishi - Department of Veterinary and Comparative Anatomy, Pharmacology and Physiology, Washington State University, Pullman, WA 99164-6520, USABálint Kacsóh - Division of Basic Medical Sciences and Department of Pediatrics, School of Medicine, Mercer University, Macon, GA 31207, USAFerenc Obál - Department of Veterinary and Comparative Anatomy, Pharmacology and Physiology, Washington State University, Pullman, WA 99164-6520, USAJames M Krueger - Department of Veterinary and Comparative Anatomy, Pharmacology and Physiology, Washington State University, Pullman, WA 99164-6520, USA
- Publication Details
- Peptides (New York, N.Y. : 1980), Vol.23(8), pp.1497-1502
- Academic Unit
- Integrative Physiology and Neuroscience, Department of
- Publisher
- Elsevier Inc
- Identifiers
- 99900547176701842
- Language
- English
- Resource Type
- Journal article