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CC BY V4.0, Open Access
Journal article
Alterations in sperm DNA methylation, non-coding RNA and histone retention associate with DDT-induced epigenetic transgenerational inheritance of disease
Epigenetics & chromatin, Vol.11(1), pp.8-8
02/27/2018
Handle:
https://hdl.handle.net/2376/116447
PMCID: PMC5827984
PMID: 29482626
Abstract
Environmental toxicants such as DDT have been shown to induce the epigenetic transgenerational inheritance of disease (e.g., obesity) through the germline. The current study was designed to investigate the DDT-induced concurrent alterations of a number of different epigenetic processes including DNA methylation, non-coding RNA (ncRNA) and histone retention in sperm.
Gestating females were exposed transiently to DDT during fetal gonadal development, and then, the directly exposed F1 generation, the directly exposed germline F2 generation and the transgenerational F3 generation sperm were investigated.
DNA methylation and ncRNA were altered in each generation sperm with the direct exposure F1 and F2 generations being predominantly distinct from the F3 generation epimutations. The piRNA and small tRNA were the most predominant classes of ncRNA altered. A highly conserved set of histone retention sites were found in the control lineage generations which was not significantly altered between generations, but a large number of new histone retention sites were found only in the transgenerational generation DDT lineage sperm.
Therefore, all three different epigenetic processes were concurrently altered as DDT induced the epigenetic transgenerational inheritance of sperm epimutations. The direct exposure generations sperm epigenetic alterations were distinct from the transgenerational sperm epimutations. The genomic features and gene associations with the epimutations were investigated to help elucidate the integration of these different epigenetic processes. Observations demonstrate all three epigenetic processes are involved in transgenerational inheritance. The different epigenetic processes appear to be integrated in mediating the epigenetic transgenerational inheritance phenomenon.
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Details
- Title
- Alterations in sperm DNA methylation, non-coding RNA and histone retention associate with DDT-induced epigenetic transgenerational inheritance of disease
- Creators
- Michael K Skinner - Center for Reproductive Biology, School of Biological Sciences, Washington State University, Pullman, WA, 99164-4236, USA. skinner@wsu.eduMillissia Ben Maamar - Center for Reproductive Biology, School of Biological Sciences, Washington State University, Pullman, WA, 99164-4236, USAIngrid Sadler-Riggleman - Center for Reproductive Biology, School of Biological Sciences, Washington State University, Pullman, WA, 99164-4236, USADaniel Beck - Center for Reproductive Biology, School of Biological Sciences, Washington State University, Pullman, WA, 99164-4236, USAEric Nilsson - Center for Reproductive Biology, School of Biological Sciences, Washington State University, Pullman, WA, 99164-4236, USAMargaux McBirney - Center for Reproductive Biology, School of Biological Sciences, Washington State University, Pullman, WA, 99164-4236, USARachel Klukovich - Department of Physiology and Cell Biology, University of Nevada, Reno School of Medicine, Reno, NV, 89557, USAYeming Xie - Department of Physiology and Cell Biology, University of Nevada, Reno School of Medicine, Reno, NV, 89557, USAChong Tang - Department of Physiology and Cell Biology, University of Nevada, Reno School of Medicine, Reno, NV, 89557, USAWei Yan - Department of Physiology and Cell Biology, University of Nevada, Reno School of Medicine, Reno, NV, 89557, USA
- Publication Details
- Epigenetics & chromatin, Vol.11(1), pp.8-8
- Academic Unit
- Biological Sciences, School of
- Publisher
- England
- Grant note
- 50183 / John Templeton Foundation R01 HD085506 / NICHD NIH HHS ES012974 / National Institutes of Health R01 ES012974 / NIEHS NIH HHS HD085506 / National Institutes of Health GM110767 / National Institutes of Health P30 GM110767 / NIGMS NIH HHS
- Identifiers
- 99900547644401842
- Language
- English
- Resource Type
- Journal article