Journal article
Alterations in the ovarian transcriptome during primordial follicle assembly and development
Biology of reproduction, Vol.72(1), pp.241-255
2005
Handle:
https://hdl.handle.net/2376/116012
PMID: 15371273
Abstract
The assembly of the developmentally arrested primordial fol-licle and subsequent transition to the primary follicle are poorly understood processes critical to ovarian biology. Abnormal pri-mordial follicle development can lead to pathologies such as premature ovarian failure. The current study used a genome-wide expression profile to investigate primordial follicle assembly and development. Rat ovaries with predominantly unassem-bled, primordial, or primary follicles were obtained. RNA from these ovaries was hybridized to rat microarray gene chips, and the gene expression (i.e., ovarian transcriptome) was compared between the developmental stages. Analysis of the ovarian tran-scriptome demonstrated 148 genes up-regulated and 50 genes down-regulated between the unassembled and primordial folli-cle stages. Observations demonstrate 80 genes up-regulated and 44 genes down-regulated between the primordial and primary follicle stages. The analysis demonstrated 2332 genes common among the three developmental stages, 146 genes specific for the unassembled follicles, 94 genes specific for the primordial follicles, and 151 genes specific for the primary follicles. Ste-roidogenic genes are up-regulated between unassembled and primordial follicles, and then many are again down-regulated between primordial and primary follicles. The hormones inhibin and Mü llerian inhibitory substance (MIS) display a similar pattern of expression with the highest levels of mRNA in the pri-mordial follicles. Several novel unknown genes that had dramatic changes in expression during primordial follicle development were also identified. Gene families/clusters identified that were up-regulated from unassembled to primordial follicles include growth factors and signal transduction gene clusters, whereas a down-regulated gene family was the synaptonemal complex genes associated with meiosis. Gene families/clusters that were up-regulated between primordial and primary follicles included immune response genes, metabolic enzymes, and pro-teases, whereas down-regulated gene families include the glob-ulin genes and some steroidogenic genes. The expression of several growth factors changed during primordial follicle development , including vascular endothelial growth factor and insulin-like growth factor II. Elucidation of how these changes in gene expression coordinate primordial follicle assembly and the pri-mordial to primary follicle transition provides a better understanding of these critical biological processes and allows selection of candidate regulatory factors for further investigation.
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Details
- Title
- Alterations in the ovarian transcriptome during primordial follicle assembly and development
- Creators
- Phillip R KEZELE - Center for Reproductive Biology, School of Molecular Biosciences, Washington State University, Pullman, Washington 99164-4231, United StatesJacquelyn M AGUE - Center for Reproductive Biology, School of Molecular Biosciences, Washington State University, Pullman, Washington 99164-4231, United StatesEric NILSSON - Center for Reproductive Biology, School of Molecular Biosciences, Washington State University, Pullman, Washington 99164-4231, United StatesMichael K SKINNER - Center for Reproductive Biology, School of Molecular Biosciences, Washington State University, Pullman, Washington 99164-4231, United States
- Publication Details
- Biology of reproduction, Vol.72(1), pp.241-255
- Academic Unit
- Biological Sciences, School of
- Publisher
- Society for the Study of Reproduction; Madison, WI
- Identifiers
- 99900547905001842
- Language
- English
- Resource Type
- Journal article