Journal article
Altered Testicular Gene Expression Patterns in Mice Lacking the Polyubiquitin Gene Ubb
Molecular reproduction and development, Vol.78(6), pp.415-425
06/2011
Handle:
https://hdl.handle.net/2376/115119
PMCID: PMC3133727
PMID: 21542049
Abstract
Ubiquitin (Ub) is an essential protein found in all eukaryotic cells and plays important roles in a variety of cellular functions including germ cell development. We have previously reported that targeted disruption of the polyubiquitin gene
Ubb
results in male and female infertility in
Ubb
−/−
mice, with germ cells arrested at meiotic prophase I. Although reduced Ub levels in germ cells are believed to be responsible for the fertility defect in
Ubb
−/−
mice, it is still unclear how reduced Ub levels result in sterility. Here we describe the results of a microarray analysis of the murine testicular transcriptome, which demonstrates dramatically altered gene expression patterns in
Ubb
−/−
mice, possibly related to reduced levels of histone 2A (H2A) ubiquitylation. We find that large numbers of genes related to fertility, metabolism, transcription, and the ubiquitin–proteasome system (UPS) are misregulated in
Ubb
−/−
mice. Such wide-ranging alterations in gene expression suggest that loss of the
Ubb
gene does not mimic a single-gene defect phenotype, but instead may affect gene expression more globally. These dramatic changes in gene expression could, at least in part, contribute to the complex fertility and metabolic phenotypes seen in these mice.
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Details
- Title
- Altered Testicular Gene Expression Patterns in Mice Lacking the Polyubiquitin Gene Ubb
- Creators
- SHAMIM A SINNAR - Bio-X Program, Department of Biology, Stanford University, Stanford, California 94305CHRISTOPHER L SMALL - School of Molecular Biosciences, Washington State University, Pullman, Washington 99164RYAN M EVANOFF - School of Molecular Biosciences, Washington State University, Pullman, Washington 99164LAURA G REINHOLDT - The Jackson Laboratory, Bar Harbor, Maine 04609MICHAEL D GRISWOLD - School of Molecular Biosciences, Washington State University, Pullman, Washington 99164RON R KOPITO - Bio-X Program, Department of Biology, Stanford University, Stanford, California 94305KWON-YUL RYU - Department of Life Science, University of Seoul, Seoul 130-743, Republic of Korea
- Publication Details
- Molecular reproduction and development, Vol.78(6), pp.415-425
- Academic Unit
- Molecular Biosciences, School of
- Grant note
- R01 NS042842-09 || NS / National Institute of Neurological Disorders and Stroke : NINDS
- Identifiers
- 99900547423501842
- Language
- English
- Resource Type
- Journal article