Journal article
Alternative Polyadenylation Coordinates Embryonic Development, Sexual Dimorphism and Longitudinal Growth in Xenopus tropicalis
Cellular and molecular life sciences : CMLS, Vol.76(11), pp.2185-2198
06/2019
Handle:
https://hdl.handle.net/2376/116606
PMID: 30729254
Abstract
RNA alternative polyadenylation contributes to the complexity of information transfer from genome to phenome, thus amplifying gene function. Here we report the first X. tropicalis resource with 127,914 alternative polyadenylation (APA) sites derived from embryos and adults. Overall, APA networks play central roles in coordinating the maternal-to-zygotic transition (MZT) in embryos, sexual dimorphism in adults and longitudinal growth from embryos to adults. APA sites coordinate reprogramming in embryos before the MZT, but developmental events after the MZT due to zygotic genome activation. The APA transcriptomes of young adults are more variable than growing adults and male frog APA transcriptomes are more divergent than females. The APA profiles of young females were similar to embryos before the MZT. Enriched pathways in developing embryos were distinct across the MZT and noticeably segregated from adults. Briefly, our results suggest that the minimal functional units in genomes are alternative transcripts as opposed to genes.
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Details
- Title
- Alternative Polyadenylation Coordinates Embryonic Development, Sexual Dimorphism and Longitudinal Growth in Xenopus tropicalis
- Creators
- Xiang Zhou - department of Animal Sciences and Center for Reproductive Biology, Washington State University, Pullman, WA, USAYangzi Zhang - department of Animal Sciences and Center for Reproductive Biology, Washington State University, Pullman, WA, USAJennifer J Michal - department of Animal Sciences and Center for Reproductive Biology, Washington State University, Pullman, WA, USALujiang Qu - department of Animal Sciences and Center for Reproductive Biology, Washington State University, Pullman, WA, USAShuwen Zhang - department of Animal Sciences and Center for Reproductive Biology, Washington State University, Pullman, WA, USAMark R Wildung - Laboratory for Biotechnology and Bioanalysis, Center for Reproductive Biology, Washington State University, Pullman, WA, USAWeiwei Du - Laboratory for Biotechnology and Bioanalysis, Center for Reproductive Biology, Washington State University, Pullman, WA, USADerek J Pouchnik - Laboratory for Biotechnology and Bioanalysis, Center for Reproductive Biology, Washington State University, Pullman, WA, USAHui Zhao - School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, ChinaYin Xia - School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, ChinaHonghua Shi - State Key Laboratory of Estuarine and Coastal Research, East China Normal University, Shanghai, ChinaGuoli Ji - Department of Automation, Xiamen University, Xiamen, ChinaJon F Davis - Department of Integrative Physiology and Neuroscience, Washington State University, Pullman, WA, USAGary D Smith - Departments of OB/GYN, Physiology, and Urology, University of Michigan, Ann Arbor, MI, USAMichael D Griswold - Laboratory for Biotechnology and Bioanalysis, Center for Reproductive Biology, Washington State University, Pullman, WA, USARichard M Harland - Department of Molecular and Cell Biology, University of California Berkeley, Berkeley, CA, USAZhihua Jiang - department of Animal Sciences and Center for Reproductive Biology, Washington State University, Pullman, WA, USA
- Publication Details
- Cellular and molecular life sciences : CMLS, Vol.76(11), pp.2185-2198
- Academic Unit
- Molecular Biosciences, School of; Animal Sciences, Department of; Integrative Physiology and Neuroscience, Department of
- Identifiers
- 99900547615801842
- Language
- English
- Resource Type
- Journal article