Amperometric biosensors based on carbon paste electrodes modified with nanostructured mixed-valence manganese oxides and glucose oxidase

Xiaoli Cui; Guodong Liu; Yuehe Lin

doi:10.1016/j.nano.2005.03.005

Back

Amperometric biosensors based on carbon paste electrodes modified with nanostructured mixed-valence manganese oxides and glucose oxidase

Journal article

Peer reviewed

Amperometric biosensors based on carbon paste electrodes modified with nanostructured mixed-valence manganese oxides and glucose oxidase

Xiaoli Cui, Guodong Liu and Yuehe Lin

Nanomedicine, Vol.1(2), pp.130-135

06/2005

DOI: https://doi.org/10.1016/j.nano.2005.03.005

Handle:

https://hdl.handle.net/2376/115674

PMID: 17292069

Abstract

Glucose Oxidase - chemistry

Enzymes, Immobilized - chemistry

Microelectrodes

Nanostructures - ultrastructure

Reproducibility of Results

Oxides - chemistry

Coated Materials, Biocompatible - chemistry

Equipment Design

Hydrogen Peroxide - chemistry

Hydrogen Peroxide - analysis

Sensitivity and Specificity

Surface Properties

Glucose - chemistry

Nanostructures - chemistry

Biosensing Techniques - instrumentation

Biosensing Techniques - methods

Electrochemistry - methods

Glucose - analysis

Ointments

Electrochemistry - instrumentation

Equipment Failure Analysis

Manganese Compounds - chemistry

Nanostructured, multivalent, manganese-oxide octahedral molecular sieves (OMS), including cryptomelane-type manganese oxides and todorokite-type manganese oxides, were synthesized and evaluated for chemical sensing and biosensing at low operating potential. Both cryptomelane-type manganese oxides and todorokite-type manganese oxides are nanofibrous crystals with subnanometer open tunnels that provide a unique property for sensing applications. The electrochemical and electrocatalytic performance of OMS for the oxidation of H2O2 have been compared. Both cryptomelane-type manganese oxides and todorokite-type manganese oxides can be used to fabricate sensitive H2O2 sensors. With glucose oxidase (GOx) as an enzyme model, amperometric glucose biosensors are constructed by bulk modification of carbon paste electrodes with GOx as a biocomponent and nanostructured OMS as a mediator. A Nafion thin film was applied as an immobilization/encapsulation and protective layer. The biosensors were evaluated as an amperometric glucose detector at phosphate buffer solution with a pH 7.4 at an operating potential of 0.3 V (vs Ag/AgCl). The biosensor is characterized by a well-reproducible amperometric response, linear signal-to-glucose concentration range up to 3.5 mmol/L and 1.75 mmol/L, and detection limits (S/N = 3) of 0.1 mmol/L and 0.05 mmol/L for todorokite-type manganese oxide and cryptomelane-type manganese oxide-modified electrodes, respectively. The biosensors based on OMS exhibit considerable good reproducibility and stability, and the construction and renewal are simple and inexpensive.

Metrics

8 Record Views

See more details

Details

Title: Amperometric biosensors based on carbon paste electrodes modified with nanostructured mixed-valence manganese oxides and glucose oxidase
Creators: Xiaoli Cui - Pacific Northwest National Laboratory, Richland, Washington, USA
Guodong Liu
Yuehe Lin
Publication Details: Nanomedicine, Vol.1(2), pp.130-135
Academic Unit: Mechanical and Materials Engineering, School of
Publisher: United States
Identifiers: 99900548274101842
Language: English
Resource Type: Journal article

Amperometric biosensors based on carbon paste electrodes modified with nanostructured mixed-valence manganese oxides and glucose oxidase

Related links

Abstract

Metrics

Details