Journal article
Amperometric detection of triazophos pesticide using acetylcholinesterase biosensor based on multiwall carbon nanotube–chitosan matrix
Sensors and actuators. B, Chemical, Vol.127(2), pp.531-535
2007
Handle:
https://hdl.handle.net/2376/107169
Abstract
A simple method for immobilization of acetylcholinesterase (AChE) on multiwall carbon nanotubes (MWNTs)–chitosan (MC) composite was proposed and thus a sensitive, fast and stable amperometric sensor for quantitative determination of organophosphorous insecticide was developed. Atomic force microscopy showed that this matrix possessed homogeneously netlike structure, which prevented enzyme from leaving out of the electrode. MWNTs promoted electron transfer reactions at a lower potential and catalyzed the electro-oxidation of thiocholine, thus increasing detection sensitivity. Based on the inhibition of organophosphorous insecticide to the enzymatic activity of AChE, using triazophos as a model compound, the conditions for detection of the insecticide were explored. Under optimal conditions, the inhibition of triazophos was proportional to its concentration in two ranges, from 0.03 to 7.8
μM and 7.8 to 32
μM with a detection limit of 0.01
μM. A 95% reactivation of the inhibited AChE could be regenerated for using pralidoxime iodide within 8
min. The constructed biosensor processing prominent characteristics and performance such as good precision and reproducibility, acceptable stability and accuracy, fast response and low detection limit has potential application in the characterization of enzyme inhibitors and detection of toxic compounds against to enzyme.
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Details
- Title
- Amperometric detection of triazophos pesticide using acetylcholinesterase biosensor based on multiwall carbon nanotube–chitosan matrix
- Creators
- Dan Du - Key Laboratory of Pesticide & Chemical Biology of Ministry of Education, Central China Normal University, Wuhan 430079, PR ChinaXi Huang - Key Laboratory of Pesticide & Chemical Biology of Ministry of Education, Central China Normal University, Wuhan 430079, PR ChinaJie Cai - The Technology Center of Wuhan Iron & Steel Company, Wuhan 430080, PR ChinaAidong Zhang - Key Laboratory of Pesticide & Chemical Biology of Ministry of Education, Central China Normal University, Wuhan 430079, PR China
- Publication Details
- Sensors and actuators. B, Chemical, Vol.127(2), pp.531-535
- Academic Unit
- School of Mechanical and Materials Engineering
- Publisher
- Elsevier B.V
- Identifiers
- 99900547082901842
- Language
- English
- Resource Type
- Journal article