Journal article
Amperometric immunosensor for rapid detection of Mycobacterium tuberculosis
Journal of micromechanics and microengineering, Vol.25(5), pp.055013-9
04/07/2015
Handle:
https://hdl.handle.net/2376/117057
PMCID: PMC4469293
PMID: 26097292
Abstract
Tuberculosis (TB) has been a major public health problem, which can be better controlled by using accurate and rapid diagnosis in low-resource settings. A simple, portable, and sensitive detection method is required for point-of-care (POC) settings. This paper studies an amperometric biosensor using a microtip immunoassay for a rapid and low-cost detection of Mycobacterium tuberculosis (MTB) in sputum. MTB in sputum is specifically captured on the functionalized microtip surface and detected by electric current. According to the numerical study, the current signal on the microtip surface is linearly changed with increasing immersion depth. Using a reference microtip, the immersion depth is compensated for a sensing microtip. On the microtip surface, target bacteria are concentrated and organized by a coffee-ring effect, which amplifies the electric current. To enhance the signal-to-noise ratio, both the sample processing and rinsing steps are presented with the use of deionized water as a medium for the amperometric measurement. When applied to cultured MTB cells spiked into human sputum, the detection limit was 100 CFU mL−1, comparable to a more labor-intensive fluorescence detection method reported previously.
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Details
- Title
- Amperometric immunosensor for rapid detection of Mycobacterium tuberculosis
- Creators
- Morgan Hiraiwa - University of Washington Department of Mechanical Engineering, Seattle, WA 98195, USAJong-Hoon Kim - Washington State University School of Engineering and Computer Science, Vancouver, WA 98686, USAHyun-Boo Lee - University of Washington Department of Mechanical Engineering, Seattle, WA 98195, USAShinnosuke Inoue - University of Washington Department of Mechanical Engineering, Seattle, WA 98195, USAAnnie L Becker - University of Washington Department of Environmental and Occupational Health Sciences, Seattle, WA 98195, USAKris M Weigel - University of Washington Department of Environmental and Occupational Health Sciences, Seattle, WA 98195, USAGerard A Cangelosi - University of Washington Department of Environmental and Occupational Health Sciences, Seattle, WA 98195, USAKyong-Hoon Lee - NanoFacture, Inc. , 2601 151 Pl NE, Redmond, WA 98052, USAJae-Hyun Chung - University of Washington Department of Mechanical Engineering, Seattle, WA 98195, USA
- Publication Details
- Journal of micromechanics and microengineering, Vol.25(5), pp.055013-9
- Academic Unit
- Engineering and Computer Science (VANC), School of
- Publisher
- IOP Publishing
- Number of pages
- 9
- Identifiers
- 99900547838101842
- Language
- English
- Resource Type
- Journal article