Journal article
An HPLC-based fluorometric assay for serine hydroxymethyltransferase
Analytical biochemistry, Vol.375(2), pp.367-369
04/15/2008
Handle:
https://hdl.handle.net/2376/104225
PMID: 18194659
Abstract
We have developed a novel HPLC-based fluorometric assay for serine hydroxymethyltransferase activity. In this assay, the 5,10-CH(2)-H(4)PteGlu formed by serine hydroxymethyltransferase activity is reduced to 5-CH(3)-H(4)PteGlu using NaBH(4). Then the fluorescent assay components are separated by reversed-phase chromatography under isocratic conditions and 5-CH(3)-H(4)PteGlu is quantified by comparison with standards. We show that this assay can be used to measure serine hydroxymethyltransferase activity at 10(-8) to 10(-3)M (6R,S)-H(4)PteGlu.
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Details
- Title
- An HPLC-based fluorometric assay for serine hydroxymethyltransferase
- Creators
- Yi Zhang - Institute of Biological Chemistry, Washington State University, Pullman, WA 99164, USAKehan SunSanja Roje
- Publication Details
- Analytical biochemistry, Vol.375(2), pp.367-369
- Academic Unit
- Biological Chemistry, Institute of
- Publisher
- United States
- Identifiers
- 99900546657101842
- Language
- English
- Resource Type
- Journal article