Journal article
An expression cDNA library for suppression cloning in yeast mutants, complementation of a yeast his4 mutant, and EST analysis from the symbiotic basidiomycete Hebeloma cylindrosporum
Genome, Vol.46(2), pp.177-181
04/2003
Handle:
https://hdl.handle.net/2376/108695
PMID: 12723033
Abstract
An oriented expression library was constructed from the mycelia of the symbiotic model fungus Hebeloma cylindrosporum in the high-level yeast expression vector pDR196. DNA sequencing of approximately 500 expressed sequence tags (ESTs) showed that 15% correspond to known genes, two thirds contain sequences with unknown function, andthe remaining 20% showed no significant similarity to any known genes. The ESTs had a GC content between 44 and 56%, with most of them having a GC content of 52-54%, which could be correlated with GC contents of fungal genes. The library was successfully used to identify the Hebeloma HIS4 gene by functional complementation of a yeast his4 mutant. Thus, the library may serve as a powerful tool for identification and characterization of mycorrhizal genes by EST analysis and for the identification of ectomycorrhizal genes by means of suppression cloning.
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Details
- Title
- An expression cDNA library for suppression cloning in yeast mutants, complementation of a yeast his4 mutant, and EST analysis from the symbiotic basidiomycete Hebeloma cylindrosporum
- Creators
- Daniel Wipf - ZMBP, Plant Physiology, Auf der Morgenstelle 1, D-72076 Tübingen, Germany. daniel.wipf@zmbp.uni-tuebingen.deMarlam BenjdiaEnno RikirschSabine ZimmermannMechthild TegederWolf B Frommer
- Publication Details
- Genome, Vol.46(2), pp.177-181
- Academic Unit
- Biological Sciences, School of
- Publisher
- Canada
- Identifiers
- 99900546902101842
- Language
- English
- Resource Type
- Journal article