Journal article
An improved Severinghaus-type carbon dioxide microelectrode for use in biofilms
Sensors and actuators. B, Chemical, Vol.97(2), pp.202-210
2004
Handle:
https://hdl.handle.net/2376/115845
Abstract
Severinghaus-type carbon dioxide microelectrodes with a tip diameter of less than 20
μm were constructed using anodically grown iridium oxide film (AIROF) as the internal pH sensor. The AIROF, which was formed at the tip of the iridium wire by cyclic voltammetry in diluted sulfuric acid, showed a super-Nernstian response to pH changes, with a slope between 65 and 80
mV/pH. Therefore, our microelectrodes were more sensitive to changes in carbon dioxide concentration than the previously described microelectrodes, which used liquid ion exchanger (LIX) pH membranes and had a Nernstian response of 59
mV/pH. When calibrated, our microelectrodes showed 65–76
mV per decade of μatm
pCO
2, and were more sensitive than the microelectrodes using LIX membranes, which showed 57
mV per decade of μatm
pCO
2. AIROF electrodes were serviceable after 1 month of storage, showing only a modest potential drift of 0.03±0.01
mV/h. Their utility was demonstrated by measuring CO
2 concentration profiles in
Staphylococcus aureus (MN8) biofilms.
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Details
- Title
- An improved Severinghaus-type carbon dioxide microelectrode for use in biofilms
- Creators
- Haluk Beyenal - Center for Biofilm Engineering, P.O. Box 173980, Room 366 EPS, Montana State University, Bozeman, MT 59717-3980, USACatherine C Davis - Clinical Microbiology, PS&RA FemCare, The Procter & Gamble Company, WHTC, 6110 Center Hill Avenue, Cincinnati, OH 45224, USAZbigniew Lewandowski - Center for Biofilm Engineering, P.O. Box 173980, Room 366 EPS, Montana State University, Bozeman, MT 59717-3980, USA
- Publication Details
- Sensors and actuators. B, Chemical, Vol.97(2), pp.202-210
- Academic Unit
- Chemical Engineering and Bioengineering, School of
- Publisher
- Elsevier B.V
- Identifiers
- 99900548255601842
- Language
- English
- Resource Type
- Journal article