Journal article
An in vivo model to investigate lymphocyte-mediated immunity during acute hemoparasitic infections. Use of a monoclonal antibody to selectively deplete CD4+ T lymphocytes from thymectomized calves
Annals of the New York Academy of Sciences, Vol.916(1), pp.233-236
2000
Handle:
https://hdl.handle.net/2376/113010
PMID: 11193625
Abstract
Thymectomized calves were selectively depleted of CD4+ T lymphocytes with a monoclonal antibody (mAb) specific for the bovine CD4 monomer (ILA-11). Calves were treated with high loading doses of ILA-11 during the first week of the study then treated with subsequent lower maintenance doses. Depletion of CD4+ T lymphocytes was assessed weekly by flow cytometric analysis of PBMC and mononuclear cells from lymph node and spleen biopsies. Treatment with high doses of ILA-11 resulted in rapid and marked depletion of CD4+ T lymphocytes from the peripheral blood, peripheral lymph nodes, and spleen. Although CD4+ T lymphocytes slowly returned to the peripheral blood, peripheral lymph nodes, and spleen by day 21 post-treatment, the numbers of CD4+ T lymphocytes in depleted calves remained below pre-depletion levels for the duration of the study. CD4+ T lymphocytes failed to be effectively depleted from a non-thymectomized calf treated with the mAb ILA-11. Development of a T lymphocyte depletion model in thymectomized calves will permit testing of the hypothesis that CD4+ T lymphocytes and IFN-gamma are required in cattle for control of acute anaplasmosis. In subsequent planned studies, thymectomized calves depleted of CD4+ T lymphocytes will be experimentally infected with A. marginale and parameters of disease compared between depleted and non-depleted calves.
Metrics
4 Record Views
Details
- Title
- An in vivo model to investigate lymphocyte-mediated immunity during acute hemoparasitic infections. Use of a monoclonal antibody to selectively deplete CD4+ T lymphocytes from thymectomized calves
- Creators
- R A Valdez - United States Department of Agriculture, Agricultural Research Service, Animal Disease Research Unit, P.O. Box 646630, Pullman, WA 99164-6630, USA. reg@vetmed.wsu.eduT C McGuireW C BrownW C DavisD P Knowles
- Publication Details
- Annals of the New York Academy of Sciences, Vol.916(1), pp.233-236
- Academic Unit
- Veterinary Microbiology and Pathology, Department of
- Publisher
- United States
- Grant note
- K08 AI01447 / NIAID NIH HHS
- Identifiers
- 99900547687801842
- Language
- English
- Resource Type
- Journal article