Analysis of CaM-kinase signaling in cells

Gary A Wayman; Hiroshi Tokumitsu; Monika A Davare; Thomas R Soderling

doi:10.1016/j.ceca.2011.02.007

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Analysis of CaM-kinase signaling in cells

Journal article

Open access

Peer reviewed

Analysis of CaM-kinase signaling in cells

Gary A Wayman, Hiroshi Tokumitsu, Monika A Davare and Thomas R Soderling

Cell calcium (Edinburgh), Vol.50(1), pp.1-8

2011

DOI: https://doi.org/10.1016/j.ceca.2011.02.007

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https://hdl.handle.net/2376/106682

PMCID: PMC3236032

PMID: 21529938

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Abstract

Calcium

CaM kinase I

CaM kinase kinase

CaM kinase IV

Dominant negative, sh-RNA

CaM kinase II, neuron

CaM kinase

A change in intracellular free calcium is a common signaling mechanism that modulates a wide array of physiological processes in most cells. Responses to increased intracellular Ca 2+ are often mediated by the ubiquitous protein calmodulin (CaM) that upon binding Ca 2+ can interact with and alter the functionality of numerous proteins including a family of protein kinases referred to as CaM-kinases (CaMKs). Of particular interest are multifunctional CaMKs, such as CaMKI, CaMKII, CaMKIV and CaMKK, that can phosphorylate multiple downstream targets. This review will outline several protocols we have used to identify which members and/or isoforms of this CaMK family mediate specific cellular responses with a focus on studies in neurons. Many previous studies have relied on a single approach such as pharmacological inhibitors or transfected dominant-negative kinase constructs. Since each of these protocols has its limitations, that will be discussed, we emphasize the necessity to use multiple, independent approaches in mapping out cellular signaling pathways.

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Title: Analysis of CaM-kinase signaling in cells
Creators: Gary A Wayman - Program in Neuroscience, Department of Veterinary and Comparative Anatomy, Pharmacology and Physiology, Washington State University, Pullman, WA, USA
Hiroshi Tokumitsu - Department of Signal Transduction Sciences, Faculty of Medicine, Kagawa University, Kagawa, Japan
Monika A Davare - Vollum Institute, Oregon Health & Science University, Portland, OR, USA
Thomas R Soderling - Vollum Institute, Oregon Health & Science University, Portland, OR, USA
Publication Details: Cell calcium (Edinburgh), Vol.50(1), pp.1-8
Academic Unit: Integrative Physiology and Neuroscience, Department of
Publisher: Elsevier India Pvt Ltd
Identifiers: 99900546993901842
Language: English
Resource Type: Journal article