Analysis of herpes simplex virion tegument ICP4 derived from infected cells and ICP4-expressing cells

Suzanne M Pritchard; Cristina W Cunha; Anthony V Nicola

doi:10.1371/journal.pone.0070889

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Analysis of herpes simplex virion tegument ICP4 derived from infected cells and ICP4-expressing cells

Journal article

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Analysis of herpes simplex virion tegument ICP4 derived from infected cells and ICP4-expressing cells

Suzanne M Pritchard, Cristina W Cunha and Anthony V Nicola

PloS one, Vol.8(8), pp.e70889-e70889

2013

DOI: https://doi.org/10.1371/journal.pone.0070889

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https://hdl.handle.net/2376/104651

PMCID: PMC3735503

PMID: 23940659

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Abstract

Cricetinae

Chlorocebus aethiops

Cricetulus

Humans

Simplexvirus - genetics

Cells, Cultured

Virion - metabolism

Herpes Simplex - genetics

Tissue Distribution

Immediate-Early Proteins - metabolism

Herpes Simplex - virology

Animals

Immediate-Early Proteins - genetics

Herpes Simplex - pathology

Immediate-Early Proteins - analysis

Transgenes

Simplexvirus - metabolism

Vero Cells

CHO Cells

ICP4 is the major transcriptional regulatory protein of herpes simplex virus (HSV). It is expressed in infected cells with immediate early kinetics and is essential for viral growth. ICP4 is also a structural component of the virion tegument layer. Herpesviral tegument proteins exert regulatory functions important for takeover of the host cell. Tegument ICP4 has not been well characterized. We examined the ICP4 present in HSV-1 virions that were either derived from wild type infected cells or from ICP4-expressing (E5) cells infected with ICP4 deletion virus d120. Limited proteolysis demonstrated that virion-associated ICP4 from particles derived from E5 cells was indeed an internal component of the virion. A similar subset of virion structural proteins was detected in viral particles regardless of the cellular origin of ICP4. Genotypically ICP4-negative virions complemented with tegument ICP4 entered cells via a proteasome-dependent, pH-dependent pathway similar to wild type virions. In infected cells, ICP4 was distributed predominantly in intranuclear replication compartments regardless of whether it was expressed from a transgene or from the HSV genome.

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Title: Analysis of herpes simplex virion tegument ICP4 derived from infected cells and ICP4-expressing cells
Creators: Suzanne M Pritchard - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, United States of America
Cristina W Cunha
Anthony V Nicola
Publication Details: PloS one, Vol.8(8), pp.e70889-e70889
Academic Unit: Veterinary Microbiology and Pathology, Department of
Publisher: United States
Grant note: T32 AI007025 / NIAID NIH HHS AI007025 / NIAID NIH HHS R21 AI096103 / NIAID NIH HHS AI096103 / NIAID NIH HHS
Identifiers: 99900546774901842
Language: English
Resource Type: Journal article