Journal article
Analysis of human immunoglobulin-degrading cysteine proteinases of Trichomonas vaginalis
Infection and immunity, Vol.63(9), pp.3388-3395
09/1995
Handle:
https://hdl.handle.net/2376/106986
PMCID: PMC173466
PMID: 7642267
Abstract
Trichomonas vaginalis is a protozoan parasite that causes a widely distributed sexually transmitted disease (STD). Since immunoglobulin G (IgG) antibodies to specific trichomonad immunogens are found in serum and vaginal washes (VWs) from patients with trichomoniasis, a potential mechanism of immune evasion by this parasite might be the ability of T. vaginalis proteinases to degrade human immunoglobulins (Igs). Incubation of human IgG with lysates of T. vaginalis organisms resulted in time- and concentration-dependent degradation of the heavy chain. Secretory IgA was degraded similarly. Inhibitors of cysteine proteinases, when added to trichomonal lysates, abolished IgG and IgA degradation, while EDTA, a metalloproteinase inhibitor, did not. Substrate-gel electrophoresis with human IgG, IgM, or IgA copolymerized with acrylamide revealed several distinct cysteine proteinases in both lysates and culture supernatants from logarithmically growing parasites that degraded all classes of human antibodies. Trichomonal lysates and supernatants of numerous isolates tested all had Ig-degrading activity. Finally, proteolytic activity against IgG was detected in most (26 of 33; 78%) VWs from patients with trichomoniasis. In contrast, 18 of 28 (65%) VWs from women without trichomoniasis or from patients infected with other STDs had no detectable proteinases when tested in an identical manner. The other 10 of these 28 VWs (35%) had smaller amounts of detectable Ig-degrading proteinases. These differences in Ig-degrading proteinase activity between patients with and without trichomoniasis, regardless of coinfecting STDs, were statistically significant (P = 0.001). These results illustrate that T. vaginalis is capable of degrading human Igs.
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Details
- Title
- Analysis of human immunoglobulin-degrading cysteine proteinases of Trichomonas vaginalis
- Creators
- D Provenzano - Department of Microbiology, University of Texas Health Science Center, San Antonio 78284-7758, USAJ F Alderete
- Publication Details
- Infection and immunity, Vol.63(9), pp.3388-3395
- Academic Unit
- Molecular Biosciences, School of
- Publisher
- United States
- Grant note
- AI 31498 / NIAID NIH HHS AI 18768 / NIAID NIH HHS
- Identifiers
- 99900546735501842
- Language
- English
- Resource Type
- Journal article