Journal article
Analysis of monoclonal antibodies reactive with molecules upregulated or expressed only on activated lymphocytes
Veterinary immunology and immunopathology, Vol.52(4), pp.301-311
1996
Handle:
https://hdl.handle.net/2376/107221
PMID: 8896219
Abstract
Monoclonal antibodies potentially specific for antigens expressed or upregulated on activated leukocytes were selected for further analysis from the panel submitted to the third international workshop on ruminant leukocyte antigens. The kinetics of expression of these activation antigens on resting peripheral mononuclear cells (PBMC) and PBMC stimulated with concanavalin A or staphylococcal superantigen SEC1 for 4, 24 or 96 h were compared, as well as their appearance on various subsets of cells. For some of them, a molecular mass could be determined after immunoprecipitation from radio-labeled, lectin-stimulated cells. Based on the results from the clustering, kinetic studies and biochemical data, evidence was gathered for assigning two additional mAbs to cluster BoCD25 (IL-2 receptor) and two mAbs to cluster BoCD71 (transferrin receptor). Four mAbs recognized an early activation antigen predominantly expressed on γδ T cells in short-term cultures. A number of other activation antigens were further characterized.
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Details
- Title
- Analysis of monoclonal antibodies reactive with molecules upregulated or expressed only on activated lymphocytes
- Creators
- W.C Davis - Department of Veterinary Microbiology and Pathology and Animal Health Research Center, College of Veterinary Medicine, Washington State University, Pullman, WA 99164-7040, USAJ Naessens - International Livestock Research Institute, P.O. Box 30709, Nairobi, KenyaW.C Brown - Department of Veterinary Microbiology and Pathology and Animal Health Research Center, College of Veterinary Medicine, Washington State University, Pullman, WA 99164-7040, USAJ.A Ellis - Department of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Sask. S7N 0W0, CanadaM.J Hamilton - Department of Veterinary Microbiology and Pathology and Animal Health Research Center, College of Veterinary Medicine, Washington State University, Pullman, WA 99164-7040, USAG.H Cantor - Department of Veterinary Microbiology and Pathology and Animal Health Research Center, College of Veterinary Medicine, Washington State University, Pullman, WA 99164-7040, USAJ.I.R Barbosa - Unidad de Microbiologia e Inmunologia, Universidad de Leon, 24071 Leon, SpainW Ferens - Department of Microbiology, Molecular Biology, and Biochemistry, University of Idaho, Moscow, ID 83844-3052, USAG.A Bohach - Department of Microbiology, Molecular Biology, and Biochemistry, University of Idaho, Moscow, ID 83844-3052, USA
- Publication Details
- Veterinary immunology and immunopathology, Vol.52(4), pp.301-311
- Academic Unit
- Veterinary Microbiology and Pathology, Department of
- Publisher
- Elsevier B.V
- Identifiers
- 99900546999301842
- Language
- English
- Resource Type
- Journal article