Journal article
Analysis of monoclonal antibodies reactive with the porcine CD2 antigen
Veterinary immunology and immunopathology, Vol.43(1), pp.229-232
1994
Handle:
https://hdl.handle.net/2376/109185
PMID: 7531904
Abstract
As result of the First International Swine CD Workshop, six monoclonal antibodies (mAbs) (numbers 014, 023, 024, 057, 128, and 130) clustered closely to the internal standard anti-porcine CD2 mAb, MSA4. Despite the close clustering, the cluster was split into two subgroups. To further characterize the relationship between these mAbs, they were used in flow cytometry to inhibit binding of MSA4 to porcine lymphocytes. mAbs 014 (1038-8-31), 023 (MAC83), 024 (MAC80), 057 (PG168), and 128 (MSA4) completely inhibited the binding of MSA4, mAb 130 (MSA2) failed to inhibit MSA4 binding. On dual parameter flow cytometry comparing MSA2 with MSA4, all MSA2
+ cells were MSA4
+, two thirds of the MSA4
+ cells were MSA2
−. We conclude that five of the mAbs bind to the same or a closely related epitope on porcine lymphocytes. mAb 130 appears to have aberrantly clustered with the CD2 group of mAb.
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Details
- Title
- Analysis of monoclonal antibodies reactive with the porcine CD2 antigen
- Creators
- M.D Pescovitz - Indiana University, Department of Surgery and Microbiology/Immunology, Indianapolis, IN 46202, USAB Aasted - Royal Veterinary University, Frederiksberg C, DenmarkA Canals - Departamento de Sanidad Animal, INIA, Madrid, SpainJ Dominguez - Departamento de Sanidad Animal, INIA, Madrid, SpainJ.S Vizcaino - Departamento de Sanidad Animal, INIA, Madrid, SpainR Pospisil - Institute Microbiology, Prague, Czech RepublicJ Sinkora - Institute Microbiology, Prague, Czech RepublicH Salmon - Laboratoire D'Immunologie, Nouzilly Monnaie, FranceI Valpotic - Department of Biology, Veterinary Faculty, University of Zagreb, Zagreb, CroatiaW.C Davis - Department of Veterinary Microbiology and Pathology, Pullman, WA, USAS Arn - Transplantation Biology Research Center, Massachusetts General Hospital, Charlestown, MA, USAD.H Sachs - Transplantation Biology Research Center, Massachusetts General Hospital, Charlestown, MA, USAJ.K Lunney - USDA, ARA, Helminthic Diseases Laboratory, Beltsville, MD, USAF Zuckerman - College of Veterinary Medicine, Urbana, IL, USAE Weiland - Federal Research Center for Virus Disease of Animals, Tuebingen, GermanyA Saalmüller - Federal Research Center for Virus Disease of Animals, Tuebingen, Germany
- Publication Details
- Veterinary immunology and immunopathology, Vol.43(1), pp.229-232
- Academic Unit
- Veterinary Microbiology and Pathology, Department of
- Publisher
- Elsevier B.V
- Identifiers
- 99900547153301842
- Language
- English
- Resource Type
- Journal article