Journal article
Analysis of monoclonal antibodies reactive with the porcine CD4 antigen
Veterinary immunology and immunopathology, Vol.43(1), pp.233-236
1994
Handle:
https://hdl.handle.net/2376/110477
PMID: 7531905
Abstract
As result of the First International Swine CD Workshop, four monoclonal antibodies (mAb) (#002, 054, 118, and 127) clustered closely to the internal standard anti-porcine CD4 mAb, 74-12-4. To further characterize the relationship between these mAb, they were used in flow cytometry to inhibit binding of 74-12-4 to porcine lymphocytes. mAb #002 (74-12-4) and #054 (PT90) completely inhibited, while mAb #127 (10.2H2) and #118 (b38c6) partially inhibited (57% and 77% respectively), the binding of 74-12-4. Furthermore, none of the mAbs bound to a 74-12-4 negative strain of pigs. We conclude that the four mAb bind to the same, or a closely related, epitope on porcine lymphocytes.
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Details
- Title
- Analysis of monoclonal antibodies reactive with the porcine CD4 antigen
- Creators
- M.D Pescovitz - Indiana University, Department of Surgery and Microbiology/Immunology, Indianapolis, IN 46202, USAB Aasted - Royal Veterinary University, Frederiksberg C, DenmarkA Canals - Departamento de Sanidad Animal, INIA, Madrid, SpainJ Dominguez - Departamento de Sanidad Animal, INIA, Madrid, SpainJ.S Vizcaino - Departamento de Sanidad Animal, INIA, Madrid, SpainR Pospisil - Institute Microbiology, Prague, Czech RepublicI Trebichavsky - Institute Microbiology, Prague, Czech RepublicH Salmon - Laboratoire D'Immunologie, Nouzilly Monnaie, FranceI Valpotic - Department of Biology, Veterinary Faculty, University of Zagreb, Zagreb, CroatiaW.C Davis - Department of Veterinary Microbiology and Pathology, Pullman, WA, USAS Arn - Transplantation Biology Research Center, Massachusetts General Hospital, Charlestown, MA, USAD.H Sachs - Transplantation Biology Research Center, Massachusetts General Hospital, Charlestown, MA, USAJ.K Lunney - USDA, ARA, Helminthic Diseases Laboratory, Beltsville, MD, USAF Zuckerman - College of Veterinary Medicine, Urbana IL, USAE Weiland - Federal Research Center for Virus Disease of Animals, Tuebingen, GermanyA Saalmueller - Federal Research Center for Virus Disease of Animals, Tuebingen, Germany
- Publication Details
- Veterinary immunology and immunopathology, Vol.43(1), pp.233-236
- Academic Unit
- Veterinary Microbiology and Pathology, Department of
- Publisher
- Elsevier B.V
- Identifiers
- 99900547286201842
- Language
- English
- Resource Type
- Journal article