Journal article
Analysis of serum and lymphocyte surface IgM of healthy and immunodeficient horses with monoclonal antibodies
American journal of veterinary research, Vol.44(7), pp.1284-1288
07/1983
Handle:
https://hdl.handle.net/2376/115514
PMID: 6410944
Abstract
Nine monoclonal antibodies which reacted with equine immunoglobulin (Ig)M and not other equine Ig and serum proteins were prepared. Cells producing antibodies (C 1.9) which precipitated with IgM and bound to staphylococcal protein A were triple-cloned (C 1.9/3.2) and the antibodies further characterized. Monoclonal antibody C 1.9/3.2 reacted with an IgM determinant present on serum IgM from horses of several breeds. Studies with 125I-labeled IgM revealed the presence of this determinant on all IgM molecules. The monoclonal antibody enabled quantitation of IgM in presuckling foal and adult horse sera, using rocket electrophoresis. This procedure was used because presumably it gives a positive precipitation reaction over a wide range of antigen-antibody ratios. The C 1.9/3.2 monoclonal antibody recognized an exposed mu-chain determinant on live B lymphocytes, as determined by immunofluorescence. Also, IgM-containing cells could be identified in acetone-fixed frozen sections of lymphoid tissue. Sera from several other species carry the determinant identified by C 1.9/3.2, suggesting that the reagent may be useful for IgM studies in other species.
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Details
- Title
- Analysis of serum and lymphocyte surface IgM of healthy and immunodeficient horses with monoclonal antibodies
- Creators
- T C McGuireL E PerrymanW C Davis
- Publication Details
- American journal of veterinary research, Vol.44(7), pp.1284-1288
- Academic Unit
- Veterinary Microbiology and Pathology, Department of
- Publisher
- United States
- Grant note
- HD08886 / NICHD NIH HHS
- Identifiers
- 99900548190001842
- Language
- English
- Resource Type
- Journal article