Journal article
Analysis of the Arabidopsis rsr4-1/pdx1-3 Mutant Reveals the Critical Function of the PDX1 Protein Family in Metabolism, Development, and Vitamin B6 Biosynthesis[W]
The Plant cell, Vol.18(7), pp.1722-1735
07/2006
Handle:
https://hdl.handle.net/2376/105402
PMCID: PMC1488916
PMID: 16766694
Abstract
Vitamin B6 represents a highly important group of compounds ubiquitous in all living organisms. It has been demonstrated to alleviate oxidative stress and in its phosphorylated form participates as a cofactor in >100 biochemical reactions. By means of a genetic approach, we have identified a novel mutant,
rsr4-1
(for
reduced sugar response
), with aberrant root and leaf growth that requires supplementation of vitamin B6 for normal development. Cloning of the mutated gene revealed that
rsr4-1
carries a point mutation in a member of the PDX1/SOR1/SNZ (for Pyridoxine biosynthesis protein 1/Singlet oxygen resistant 1/Snooze) family that leads to reduced vitamin B6 content. Consequently, metabolism is broadly altered, mainly affecting amino acid, raffinose, and shikimate contents and trichloroacetic acid cycle constituents. Yeast two-hybrid and pull-down analyses showed that
Arabidopsis thaliana
PDX1 proteins can form oligomers. Interestingly, the mutant form of PDX1 has severely reduced capability to oligomerize, potentially suggesting that oligomerization is important for function. In summary, our results demonstrate the critical function of the PDX1 protein family for metabolism, whole-plant development, and vitamin B6 biosynthesis in higher plants.
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Details
- Title
- Analysis of the Arabidopsis rsr4-1/pdx1-3 Mutant Reveals the Critical Function of the PDX1 Protein Family in Metabolism, Development, and Vitamin B6 Biosynthesis[W]
- Creators
- Susan Wagner - Angewandte Genetik, Freie Universität Berlin, 14195 Berlin, GermanyAnne Bernhardt - Angewandte Genetik, Freie Universität Berlin, 14195 Berlin, GermanyJan Erik Leuendorf - Angewandte Genetik, Freie Universität Berlin, 14195 Berlin, GermanyChristel Drewke - Institut für Pharmazeutische Biologie, Rheinische Friedrich-Wilhelms-Universität Bonn, 53115 Bonn, GermanyAnna Lytovchenko - Max-Planck-Institut für Molekulare Pflanzenphysiologie, 14424 Potsdam, GermanyNader Mujahed - Institut für Pharmazeutische Biologie, Rheinische Friedrich-Wilhelms-Universität Bonn, 53115 Bonn, GermanyCristian Gurgui - Institut für Pharmazeutische Biologie, Rheinische Friedrich-Wilhelms-Universität Bonn, 53115 Bonn, GermanyWolf B Frommer - Department of Plant Biology, Stanford University, Stanford, California 94305Eckhard Leistner - Institut für Pharmazeutische Biologie, Rheinische Friedrich-Wilhelms-Universität Bonn, 53115 Bonn, GermanyAlisdair R Fernie - Max-Planck-Institut für Molekulare Pflanzenphysiologie, 14424 Potsdam, GermanyHanjo Hellmann - Angewandte Genetik, Freie Universität Berlin, 14195 Berlin, Germany
- Publication Details
- The Plant cell, Vol.18(7), pp.1722-1735
- Academic Unit
- Biological Sciences, School of
- Publisher
- American Society of Plant Biologists
- Identifiers
- 99900546993501842
- Language
- English
- Resource Type
- Journal article