Journal article
Analysis of the reactivity of anti-bovine CD8 monoclonal antibodies with cloned T cell lines and mouse L-cells transfected with bovine CD8
Veterinary immunology and immunopathology, Vol.27(1), pp.169-172
1991
Handle:
https://hdl.handle.net/2376/111316
PMID: 1902341
Abstract
Mouse L-cells transfected with bovine CD8 and two
Theileria parva-infected cloned T cell lines expressing bovine CD8 were used to screen the panel of ten monoclonal antibodies (mAbs) submitted to the workshop. Eight of the ten mAbs reacted with the transfectant and both the cloned T cell lines. However, two mAbs CC58 and BAT82A did not recognise the transfectant and only reacted with one of the T cell lines. Further biochemical studies that the eigth mAbs react with both homo- and heterodimeric forms of bovine CD8 whilst the two mAbs CC58 and BAT82A react with only heterodimeric forms. These data suggest that bovine DC8 is encoded by two genes as is the case in mouse and man.
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Details
- Title
- Analysis of the reactivity of anti-bovine CD8 monoclonal antibodies with cloned T cell lines and mouse L-cells transfected with bovine CD8
- Creators
- N.D MacHugh - ILRAD, Nairobi, KenyaA Bensaid - ILRAD, Nairobi, KenyaC.J Howard - IAH, Compton, UKW.C Davis - Pullman, WA, USAW.J Morrison - ILRAD, Nairobi, Kenya
- Publication Details
- Veterinary immunology and immunopathology, Vol.27(1), pp.169-172
- Academic Unit
- Veterinary Microbiology and Pathology, Department of
- Publisher
- Elsevier B.V
- Identifiers
- 99900548577001842
- Language
- English
- Resource Type
- Journal article